Synaptic Systems

Cat. No.: 168-0P

Amount: 100 µg

Price: $110.00

Background

Rab27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins play a crucial role in intracellular vesicle trafficking, particularly in the fusion of vesicles or organelles with their target membranes (1, 2). Two Rab27 isoforms, Rab27A and 27B, have been described so far.
Mutations in the RAB27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably caused by a dysfunction of melanosomes in melanocytes and lytic granules in CD8+ cytotoxic T lymphocytes (CTLs) (3). Additionally, Rab27A is located on mature insulin granules of pancreatic β-cells highlighting its role in insulin secretion (4, 5). Moreover, Rab27A is expressed in the retinal pigment epithelium and choriocapillaris, suggesting a functional role in ocular physiology (6). In patients with Griscelli syndrome caused by missense mutations in RAB27A, Rab27B expression is upregulated, partially compensating for the loss of Rab27A function. Rab27B has been found to regulate amylase secretion in parotid acinar cells, further emphasizing its role in exocytosis (7, 8).
Furthermore, a study by (9) revealed that Rab27A plays a critical role in the processing of inflammatory pain in mice and demonstrated that Rab27A is highly expressed in sensory neurons and the superficial dorsal horn of the spinal cord. Further studies have expanded the functional scope of Rab27A, revealing its involvement in renal fibrosis through the regulation of exosome secretion (10).

1 ) Izumi T et al., Meth. Enzymol. (2005)
2 ) Chen X et al., Biochem. Biophys. Res. Commun. (2004)
3 ) Jordens I et al., Pigment Cell Res. (2006)
4 ) Kasai K et al., J. Clin. Invest. (2005)
5 ) Aizawa T et al., J. Clin. Invest. (2005)
6 ) Aslan D et al., Pediatr Hematol Oncol ()
7 ) Tsuboi T et al., J. Cell. Sci. (2006)
8 ) Imai A et al., J. Cell. Sci. (2004)
9 ) Gross T et al., Cells (2020)
10 ) Liu X et al., Kidney Int (2020)

Protocols

Other products for this target

Cat. No. 168-0P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	168 003, 168 013
Remarks	This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	168-0p.pdf

Rab27A protein - 168-0P