Synaptic Systems

Cat. No.: 168 013

Amount: 50 µg

Price: $380.00

Cat. No. 168 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: 1 : 2000 gallery
Immunogen	Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Reactivity	Reacts with: rat (P23640), mouse (Q9ERI2), human (P51159). Other species not tested yet.
Specificity	Specific for rab 27A. No cross reactivity to rab 27B. K.O. validated PubMed: 25951179
Matching control protein/peptide	168-0P
Remarks	Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity.
Data sheet	168_013.pdf

References for Rab27A - 168 013

Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.
Adam F, Kauskot A, Kurowska M, Goudin N, Munoz I, Bordet JC, Huang JD, Bryckaert M, Fischer A, Borgel D, de Saint Basile G, et al.
Arteriosclerosis, thrombosis, and vascular biology (2018) : . 168 013 WB; tested species: mouse

Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs.
Lu A, Wawro P, Morgens DW, Portela F, Bassik MC, Pfeffer SR
eLife (2018) 7: . 168 013 WB; KD verified; tested species: human

UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 013 WB, Dot blot; tested species: mouse

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 013 WB; KD verified; tested species: human

Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 013 WB

Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 013 WB, IHC; KO verified

Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction.
Lovis P, Roggli E, Laybutt DR, Gattesco S, Yang JY, Widmann C, Abderrahmani A, Regazzi R
Diabetes (2008) 5710: 2728-36. 168 013 WB

Cat. No.: 168 013

Amount: 50 µg

Price: $380.00

IHC-P

Background

Rab27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins play a crucial role in intracellular vesicle trafficking, particularly in the fusion of vesicles or organelles with their target membranes (1, 2). Two Rab27 isoforms, Rab27A and 27B, have been described so far.
Mutations in the RAB27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably caused by a dysfunction of melanosomes in melanocytes and lytic granules in CD8+ cytotoxic T lymphocytes (CTLs) (3). Additionally, Rab27A is located on mature insulin granules of pancreatic β-cells highlighting its role in insulin secretion (4, 5). Moreover, Rab27A is expressed in the retinal pigment epithelium and choriocapillaris, suggesting a functional role in ocular physiology (6). In patients with Griscelli syndrome caused by missense mutations in RAB27A, Rab27B expression is upregulated, partially compensating for the loss of Rab27A function. Rab27B has been found to regulate amylase secretion in parotid acinar cells, further emphasizing its role in exocytosis (7, 8).
Furthermore, a study by (9) revealed that Rab27A plays a critical role in the processing of inflammatory pain in mice and demonstrated that Rab27A is highly expressed in sensory neurons and the superficial dorsal horn of the spinal cord. Further studies have expanded the functional scope of Rab27A, revealing its involvement in renal fibrosis through the regulation of exosome secretion (10).

1 ) Izumi T et al., Meth. Enzymol. (2005)
2 ) Chen X et al., Biochem. Biophys. Res. Commun. (2004)
3 ) Jordens I et al., Pigment Cell Res. (2006)
4 ) Kasai K et al., J. Clin. Invest. (2005)
5 ) Aizawa T et al., J. Clin. Invest. (2005)
6 ) Aslan D et al., Pediatr Hematol Oncol ()
7 ) Tsuboi T et al., J. Cell. Sci. (2006)
8 ) Imai A et al., J. Cell. Sci. (2004)
9 ) Gross T et al., Cells (2020)
10 ) Liu X et al., Kidney Int (2020)

Protocols

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Rab27A antibody - 168 013 K.O.

References for Rab27A - 168 013