Synaptic Systems

Cat. No.: 168 013

Amount: 50 µg

Price: $375.00

Cat. No. 168 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Reactivity	Reacts with: rat (P23640), mouse (Q9ERI2), human (P51159). Other species not tested yet.
Specificity	Specific for rab 27A. No cross reactivity to rab 27B. K.O. validated PubMed: 25951179
Matching control protein/peptide	168-0P
Remarks	Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity.
Data sheet	168_013.pdf

References for Rab27A - 168 013

Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.
Adam F, Kauskot A, Kurowska M, Goudin N, Munoz I, Bordet JC, Huang JD, Bryckaert M, Fischer A, Borgel D, de Saint Basile G, et al.
Arteriosclerosis, thrombosis, and vascular biology (2018) : . 168 013 WB; tested species: mouse

Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs.
Lu A, Wawro P, Morgens DW, Portela F, Bassik MC, Pfeffer SR
eLife (2018) 7: . 168 013 WB; KD verified; tested species: human

UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 013 WB, Dot blot; tested species: mouse

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 013 WB; KD verified; tested species: human

Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 013 WB

Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 013 WB, IHC; KO verified

Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction.
Lovis P, Roggli E, Laybutt DR, Gattesco S, Yang JY, Widmann C, Abderrahmani A, Regazzi R
Diabetes (2008) 5710: 2728-36. 168 013 WB

Rab27a Contributes to the Processing of Inflammatory Pain in Mice.
Gross T, Wack G, Syhr KMJ, Tolmachova T, Seabra MC, Geisslinger G, Niederberger E, Schmidtko A, Kallenborn-Gerhardt W
Cells (2020) 96: . 168 013 IHC; tested species: mouse

Cat. No.: 168 013

Amount: 50 µg

Price: $375.00

Background

Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.

1 ) Mahoney TR et al., Mol. Biol. Cell (2006)
2 ) Jordens I et al., Pigment Cell Res. (2006)
3 ) Tsuboi T et al., J. Cell. Sci. (2006)
4 ) Aslan D et al., Pediatr Hematol Oncol ()
5 ) Izumi T et al., Meth. Enzymol. (2005)
6 ) Kasai K et al., J. Clin. Invest. (2005)
7 ) Aizawa T et al., J. Clin. Invest. (2005)
8 ) Chen X et al., Biochem. Biophys. Res. Commun. (2004)
9 ) Westbroek W et al., Pigment Cell Res. (2004)
10 ) Imai A et al., J. Cell. Sci. (2004)

Protocols

Standard protocol

OTHER

Preabsorbtion

Other products for this target

Rab27A antibody - 168 013 K.O.

References for Rab27A - 168 013