Synaptic Systems - Homer1b/c

Cat. No.: 160 022

Amount: 200 µl

Price: $355.00

Cat. No. 160 022	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the c-terminal half of human Homer 1b (UniProt Id: Q86YM7-1)
Reactivity	Reacts with: rat (Q9Z214), mouse, human (Q86YM7-1). Other species not tested yet.
Specificity	Specific for homer 1b and 1c; no cross-reactivity to homer 1a.
Matching control protein/peptide	160-02P
Data sheet	160_022.pdf

References for Homer1b/c - 160 022

Immune activation during pregnancy exacerbates ASD-related alterations in Shank3-deficient mice.
Atanasova E, Arévalo AP, Graf I, Zhang R, Bockmann J, Lutz AK, Boeckers TM
Molecular autism (2023) 141: 1. 160 022 WB; tested species: mouse

Activation of the medial preoptic area (MPOA) ameliorates loss of maternal behavior in a Shank2 mouse model for autism.
Grabrucker S, Pagano J, Schweizer J, Urrutia-Ruiz C, Schön M, Thome K, Ehret G, Grabrucker AM, Zhang R, Hengerer B, Bockmann J, et al.
The EMBO journal (2021) : e104267. 160 022 WB; tested species: mouse

Altered Behaviors and Impaired Synaptic Function in a Novel Rat Model With a Complete Shank3 Deletion.
Song TJ, Lan XY, Wei MP, Zhai FJ, Boeckers TM, Wang JN, Yuan S, Jin MY, Xie YF, Dang WW, Zhang C, et al.
Frontiers in cellular neuroscience (2019) 13: 111. 160 022 WB; tested species: rat

Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice.
Vicidomini C, Ponzoni L, Lim D, Schmeisser MJ, Reim D, Morello N, Orellana D, Tozzi A, Durante V, Scalmani P, Mantegazza M, et al.
Molecular psychiatry (2017) 225: 689-702. 160 022 WB; tested species: mouse

Adult restoration of Shank3 expression rescues selective autistic-like phenotypes.
Mei Y, Monteiro P, Zhou Y, Kim JA, Gao X, Fu Z, Feng G
Nature (2016) 5307591: 481-4. 160 022 WB; tested species: mouse

Expression Analysis of Zinc Transporters in Nervous Tissue Cells Reveals Neuronal and Synaptic Localization of ZIP4.
De Benedictis CA, Haffke C, Hagmeyer S, Sauer AK, Grabrucker AM
International journal of molecular sciences (2021) 229: . 160 022 ICC, IHC; tested species: mouse

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 160 022 ICC

Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons.
Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM, et al.
Frontiers in cellular neuroscience (2015) 9: 496. 160 022 ICC; tested species: rat

Shank2/3 double knockout-based screening of cortical subregions links the retrosplenial area to the loss of social memory in autism spectrum disorders.
Garrido D, Beretta S, Grabrucker S, Bauer HF, Bayer D, Sala C, Verpelli C, Roselli F, Bockmann J, Proepper C, Catanese A, et al.
Molecular psychiatry (2022) : . 160 022 IHC; tested species: mouse

Cat. No.: 160 022

Amount: 200 µl

Price: $355.00

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 160 022 ICC

ICC

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)
6 ) Ciruela F et al., Mol. Cell. Neurosci. (2000)
7 ) Xiao B et al., Curr. Opin. Neurobiol. (2000)
8 ) Tu JC et al., Neuron (1999)
9 ) Brakeman PR et al., Nature (1997)
10 ) Shiraishi Y et al., J. Comp. Neurol. (2004)

Protocols

ICC

OTHER

Preabsorbtion

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Homer1b/c antibody - 160 022

References for Homer1b/c - 160 022