Synaptic Systems

Cat. No.: 153 011

Amount: 100 µg

Price: $415.00

Cat. No. 153 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: yes IHC: not tested yet IHC-P: 1 : 200 up to 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	AD2.38
Subtype	IgG1 (λ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 422 from mouse Pax6 (UniProt Id: P63015)
Epitop	AA 4 to 130 from mouse Pax6 (UniProt Id: P63015)
Reactivity	Reacts with: human (P26367), rat (P63016), mouse (P63015), chicken. Other species not tested yet.
Specificity	K.O. validated PubMed: 10409504
Remarks	WB: Nuclear extracts from tissues should be used for Western blot experiments to increase the concentration of Pax 6. IHC-P: Antigen retrieval at pH 9.0 (Tris-EDTA) for 30min at 97°C
Data sheet	153_011.pdf

References for Pax6 - 153 011

Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) (1999) 12616: 3585-96. 153 011 WB, IHC; KO verified; tested species: mouse

Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors.
Zhang T, Liu T, Hassan BA
STAR protocols (2021) 23: 100760. 153 011 ICC; tested species: mouse

IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Martins S, Müller-Schiffmann A, Erichsen L, Bohndorf M, Wruck W, Sleegers K, Van Broeckhoven C, Korth C, Adjaye J
International journal of molecular sciences (2020) 2112: . 153 011 ICC; tested species: human

Mutations in the Heterotopia Gene Eml1/EML1 Severely Disrupt the Formation of Primary Cilia.
Uzquiano A, Cifuentes-Diaz C, Jabali A, Romero DM, Houllier A, Dingli F, Maillard C, Boland A, Deleuze JF, Loew D, Mancini GMS, et al.
Cell reports (2019) 286: 1596-1611.e10. 153 011 ICC; tested species: mouse

Lymphoblast-derived integration-free iPSC lines from a female and male Alzheimer's disease patient expressing different copy numbers of a coding CNV in the Alzheimer risk gene CR1.
Schröter F, Sleegers K, Van Cauwenberghe C, Bohndorf M, Wruck W, Van Broeckhoven C, Adjaye J
Stem cell research (2016) 173: 560-563. 153 011 ICC

Lymphoblast-derived integration-free iPS cell line from a female 67-year-old Alzheimer's disease patient with TREM2 (R47H) missense mutation.
Schröter F, Sleegers K, Cuyvers E, Bohndorf M, Wruck W, Van Broeckhoven C, Adjaye J
Stem cell research (2016) 173: 553-555. 153 011 ICC

Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum.
Zhang T, Liu T, Mora N, Guegan J, Bertrand M, Contreras X, Hansen AH, Streicher C, Anderle M, Danda N, Tiberi L, et al.
Cell reports (2021) 3510: 109208. 153 011 IHC; tested species: mouse

Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female.
Martins S, Bohndorf M, Schröter F, Assar F, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research (2018) 26: 76-79. 153 011 IHC; tested species: human

Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain.
Vasudevan A, Long JE, Crandall JE, Rubenstein JL, Bhide PG
Nature neuroscience (2008) 114: 429-39. 153 011 IHC

Cat. No.: 153 011

Amount: 100 µg

Price: $415.00

Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors.
Zhang T, Liu T, Hassan BA
STAR protocols (2021) 23: 100760. 153 011 ICC; tested species: mouse

IHC-P

Background

Pax 6 (Sey) proteins regulate transcription and are composed of two DNA binding motives, an N-terminal paired domain (PD) and a C-terminal homeodomain (HD).
Mutations or deletions in the Pax 6 gene cause severe defects in the development of the eye and the central nervous system (CNS). The Pax 6 mRNA is alternatively spliced at position 47 and is translated into two proteins of 46 and 48 kDa. The amino acid sequence and basic regulatory mechanisms of Pax 6 are conserved from invertebrates to mammals.

1 ) Gehring WJ et al., Trends Genet. (1999)
2 ) Callaerts P et al., Annu. Rev. Neurosci. (1997)
3 ) Bhat SP et al., Dev. Neurosci. (2004)
4 ) Jiménez D et al., J. Neurocytol. ()

Protocols

ICC

IHC-P

Pax6 antibody - 153 011 K.O.

References for Pax6 - 153 011