Tailor-made Antibodies
and Tools for Life Science

Pax6 antibody - 153 011 K.O.

Pax6 is a transcription factor involved in the regulation of eye and brain development
Mouse monoclonal purified IgG
Cat. No.: 153 011
Amount: 100 µg
Price: $420.00
Cat. No. 153 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: yes
IHC: not tested yet
IHC-P: 1 : 200 up to 1 : 500 (see remarks) gallery  
Clone AD2.38
Subtype IgG1 (λ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 422 from mouse Pax6 (UniProt Id: P63015)
Epitop AA 4 to 130 from mouse Pax6 (UniProt Id: P63015)
Reactivity Reacts with: human (P26367), rat (P63016), mouse (P63015), chicken.
Other species not tested yet.
Specificity K.O. validated PubMed: 10409504
Remarks

WB: Nuclear extracts from tissues should be used for Western blot experiments to increase the concentration of Pax 6.
IHC-P: Antigen retrieval at pH 9.0 (Tris-EDTA) for 30min at 97°C

Data sheet 153_011.pdf

References for Pax6 - 153 011

Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) (1999) 12616: 3585-96. 153 011 WB, IHC; KO verified; tested species: mouse
Cat. No.: 153 011
Amount: 100 µg
Price: $420.00