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Pax 6 - 153 011

A transcription factor involved in the regulation of eye and brain development
Monoclonal mouse purified IgG
Cat. No.: 153 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 153 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: yes
IHC: yes
IHC-P/FFPE: 1 : 200 up to 1 : 500 (see remarks) gallery  
Clone AD2.38
Subtype IgG1 (λ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 422 from mouse Pax6 (UniProt Id: P63015)
Epitop Epitop: AA 4 to 130 from mouse Pax6 (UniProt Id: P63015)
Reactivity Reacts with: human (P26367), rat (P63016), mouse (P63015), chicken.
Other species not tested yet.
Specificity Specific for Pax 6. K.O. PubMed: 10409504
Remarks

Nuclear extracts from tissues should be used for Western blot experiments to increase the concentration of Pax 6.
IHC-P: Antigen retrieval at pH 9.0 (Tris-EDTA) for 30min at 97°C

Data sheet 153_011.pdf

References for Pax 6 - 153 011

Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) (1999) 12616: 3585-96. 153 011 WB, IHC; KO verified; tested species: mouse
IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Martins S, Müller-Schiffmann A, Erichsen L, Bohndorf M, Wruck W, Sleegers K, Van Broeckhoven C, Korth C, Adjaye J
International journal of molecular sciences (2020) 2112: . 153 011 ICC; tested species: human
Mutations in the Heterotopia Gene Eml1/EML1 Severely Disrupt the Formation of Primary Cilia.
Uzquiano A, Cifuentes-Diaz C, Jabali A, Romero DM, Houllier A, Dingli F, Maillard C, Boland A, Deleuze JF, Loew D, Mancini GMS, et al.
Cell reports (2019) 286: 1596-1611.e10. 153 011 ICC; tested species: mouse
Lymphoblast-derived integration-free iPSC lines from a female and male Alzheimer's disease patient expressing different copy numbers of a coding CNV in the Alzheimer risk gene CR1.
Schröter F, Sleegers K, Van Cauwenberghe C, Bohndorf M, Wruck W, Van Broeckhoven C, Adjaye J
Stem cell research (2016) 173: 560-563. 153 011 ICC
Lymphoblast-derived integration-free iPS cell line from a female 67-year-old Alzheimer's disease patient with TREM2 (R47H) missense mutation.
Schröter F, Sleegers K, Cuyvers E, Bohndorf M, Wruck W, Van Broeckhoven C, Adjaye J
Stem cell research (2016) 173: 553-555. 153 011 ICC
Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female.
Martins S, Bohndorf M, Schröter F, Assar F, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research (2018) 26: 76-79. 153 011 IHC; tested species: human
Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain.
Vasudevan A, Long JE, Crandall JE, Rubenstein JL, Bhide PG
Nature neuroscience (2008) 114: 429-39. 153 011 IHC
Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) (1999) 12616: 3585-96. 153 011 WB, IHC; KO verified; tested species: mouse
Cat. No.: 153 011
Quantity: 100 µg
Price: 415.00 €
Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) (1999) 12616: 3585-96. 153 011 WB, IHC; KO verified; tested species: mouse
IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Martins S, Müller-Schiffmann A, Erichsen L, Bohndorf M, Wruck W, Sleegers K, Van Broeckhoven C, Korth C, Adjaye J
International journal of molecular sciences (2020) 2112: . 153 011 ICC; tested species: human
Mutations in the Heterotopia Gene Eml1/EML1 Severely Disrupt the Formation of Primary Cilia.
Uzquiano A, Cifuentes-Diaz C, Jabali A, Romero DM, Houllier A, Dingli F, Maillard C, Boland A, Deleuze JF, Loew D, Mancini GMS, et al.
Cell reports (2019) 286: 1596-1611.e10. 153 011 ICC; tested species: mouse
Lymphoblast-derived integration-free iPSC lines from a female and male Alzheimer's disease patient expressing different copy numbers of a coding CNV in the Alzheimer risk gene CR1.
Schröter F, Sleegers K, Van Cauwenberghe C, Bohndorf M, Wruck W, Van Broeckhoven C, Adjaye J
Stem cell research (2016) 173: 560-563. 153 011 ICC
Lymphoblast-derived integration-free iPS cell line from a female 67-year-old Alzheimer's disease patient with TREM2 (R47H) missense mutation.
Schröter F, Sleegers K, Cuyvers E, Bohndorf M, Wruck W, Van Broeckhoven C, Adjaye J
Stem cell research (2016) 173: 553-555. 153 011 ICC
Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female.
Martins S, Bohndorf M, Schröter F, Assar F, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research (2018) 26: 76-79. 153 011 IHC; tested species: human
Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain.
Vasudevan A, Long JE, Crandall JE, Rubenstein JL, Bhide PG
Nature neuroscience (2008) 114: 429-39. 153 011 IHC
Role of Pax6 in development of the cerebellar system.
Engelkamp D, Rashbass P, Seawright A, van Heyningen V
Development (Cambridge, England) (1999) 12616: 3585-96. 153 011 WB, IHC; KO verified; tested species: mouse
Background
Pax 6 (Sey) proteins regulate transcription and are composed of two DNA binding motives, an N-terminal paired domain (PD) and a C-terminal homeodomain (HD).
Mutations or deletions in the Pax 6 gene cause severe defects in the development of the eye and the central nervous system (CNS). The Pax 6 mRNA is alternatively spliced at position 47 and is translated into two proteins of 46 and 48 kDa. The amino acid sequence and basic regulatory mechanisms of Pax 6 are conserved from invertebrates to mammals.