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Gephyrin - 147 208

A protein associated with inhibitory neurotransmitter receptors
Monoclonal Recombinant rat purified IgG
Cat. No.: 147 208
Amount: 50 µg
Price: 415.00 €
Cat. No. 147 208 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone RtmAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rat specific sequences. The antibody can therefore be used with standard anti-rat secondary reagents.
The antibody has been expressed in mammalian cells.

WB: Clone 3B11 (cat. no. 147 111) is highly recommended.

IP: Clone 3B11 (cat. no. 147 111) highly recommended.

Data sheet 147_208.pdf
Cat. No.: 147 208
Quantity: 50 µg
Price: 415.00 €
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

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