|Cat. No. 147 318||200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 200 gallery
IHC: 1 : 200 (see remarks) gallery
IHC-P/FFPE: not tested yet
|Subtype||IgG2 (κ light chain)|
|Immunogen||Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)|
Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
|Specificity||Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O.|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.