Synaptic Systems - Gephyrin

Cat. No.: 147 318

Amount: 200 µl

Price: $425.00

Cat. No. 147 318	200 µl purified recombinant IgG supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended (see remarks) IP: not recommended (see remarks) ICC: 1 : 200 gallery IHC: 1 : 200 (see remarks) gallery IHC-P (FFPE): not recommended IHC-Fr: 1 : 200 up to 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	GpmAb7a
Subtype	IgG2 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop	AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity	Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish. Other species not tested yet.
Specificity	Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known clone mAb7a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain and has been expressed in mammalian cells. WB: Clone 3B11 (cat. no. 147 111) is highly recommended. IP: Clone 3B11 (cat. no. 147 111) highly recommended. IHC: Antigen retrieval with citrate buffer pH 6 can be applied to improve the signal to noise ratio. Alternatively, the mild fixation protocol according to in Schneider Gasser et al. 2006, can be applied. Clone mAb7a and its recombinant derivatives can cause non-specific nuclear staining, which becomes more pronounced after AGR treatment. The culture supernatants (147 021, 147 318 and 147 018) are less prone to this effect and are the preferred choices for IHC experiments. IHC-Fr: The following fixatives are possible: 4% formaldehyde/PFA, acetone, methanol-acetone, methanol. Signal intensities as follows: PFA = acetone > acetone-methanol > methanol.
Data sheet	Datasheet 147_318

References for Gephyrin - 147 318

Distinct mechanisms control the specific synaptic functions of Neuroligin 1 and Neuroligin 2.
Wang J, Sudhof T, Wernig M
EMBO reports (2025) : . 147 318 ICC; tested species: mouse

Necl-4/CADM4 regulates GABAergic synaptic strength on GABAergic inhibitory neurons via ErbB4 activation and prevents neuronal impairments.
Komaki R, Shiotani H, Kuriu T, Kameyama T, Miyata M, Kedashiro S, Katanazaka K, Nishii S, Chihara N, Matsumoto R, Koebis M, et al.
Molecular neurobiology (2025) : . 147 318 ICC, IHC; tested species: mouse

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 147 318 ICC; tested species: mouse

Cnksr2 Loss in Mice Leads to Increased Neural Activity and Behavioral Phenotypes of Epilepsy-Aphasia Syndrome.
Erata E, Gao Y, Purkey AM, Soderblom EJ, McNamara JO, Soderling SH
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4146: 9633-9649. 147 318 ICC; tested species: mouse

Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 147 318 ICC; tested species: mouse

Stathmin 1 regulates epileptic seizures by influencing GABAA R-mediated inhibitory synaptic activity.
Yang M, Hu Y, Wu J, Zhang H, Li J, Wang X, Xu X
Neurobiology of disease (2026) 220: 107310. 147 318 IHC; tested species: human

Insulin resistance alters cortical inhibitory neurons and microglia to exacerbate Alzheimer's knock-in mouse phenotypes.
Nicholson L, Tang SJ, Karra T, Abouelatta H, Strittmatter SM
bioRxiv : the preprint server for biology (2025) : . 147 318 IHC; tested species: mouse

Semaphorin 3A-mediated perineuronal nets formation incubates depressive-like behaviors in male mice via activating parvalbumin-expressing interneurons.
Mei YX, Chen HS, Cao QL, Lv WW, Liu Y, Deng SL, Zhou Q, Zhang ZJ, Wang F, Hu ZL, Chen JG, et al.
Molecular psychiatry (2025) : . 147 318 IHC; tested species: mouse

Dual lineage origins contribute to neocortical astrocyte diversity.
Zhou J, Vitali I, Roig-Puiggros S, Javed A, Cantando I, Puglisi M, Bezzi P, Jabaudon D, Mayer C, Bocchi R
Nature communications (2025) 161: 6992. 147 318 IHC; tested species: mouse

Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.
Fang W, Chen X, He J
Neurobiology of stress (2024) 33: 100680. 147 318 IHC; tested species: mouse

Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice.
Brito DVC, Kupke J, Sokolov R, Cambridge S, Both M, Bengtson CP, Rozov A, Oliveira AMM
Molecular psychiatry (2024) : . 147 318 IHC; tested species: mouse

A thalamic-primary auditory cortex circuit mediates resilience to stress.
Li HY, Zhu MZ, Yuan XR, Guo ZX, Pan YD, Li YQ, Zhu XH
Cell (2023) 1867: 1352-1368.e18. 147 318 IHC; tested species: mouse

Neuronal activation of Gαq EGL-30/GNAQ late in life rejuvenates cognition across species.
Stevenson ME, Bieri G, Kaletsky R, St Ange J, Remesal L, Pratt KJB, Zhou S, Weng Y, Murphy CT, Villeda SA
Cell reports (2023) 429: 113151. 147 318 IHC; tested species: mouse

Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 147 318 IHC; tested species: rat

Gabrb3 is required for the functional integration of pyramidal neuron subtypes in the somatosensory cortex.
Babij R, Ferrer C, Donatelle A, Wacks S, Buch AM, Niemeyer JE, Ma H, Duan ZRS, Fetcho RN, Che A, Otsuka T, et al.
Neuron (2022) : . 147 318 IHC; tested species: mouse

Neurotransmitter phenotype switching by spinal excitatory interneurons regulates locomotor recovery after spinal cord injury.
Bertels H, Vicente-Ortiz G, El Kanbi K, Takeoka A
Nature neuroscience (2022) 255: 617-629. 147 318 IHC; tested species: mouse

Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 147 318 IHC; tested species: mouse

Cx3Cr1-Cre induction leads to microglial activation and IFN-1 signaling caused by DNA damage in early postnatal brain.
Sahasrabuddhe V, Ghosh HS
Cell reports (2022) 383: 110252. 147 318 IHC; tested species: mouse

4E-BP2-dependent translation in cerebellar Purkinje cells controls spatial memory but not autism-like behaviors.
Hooshmandi M, Truong VT, Fields E, Thomas RE, Wong C, Sharma V, Gantois I, Soriano Roque P, Chalkiadaki K, Wu N, Chakraborty A, et al.
Cell reports (2021) 354: 109036. 147 318 IHC; tested species: mouse

Morphological assessment of GABA and glutamate inputs to GnRH neurons in intact female mice using expansion microscopy.
Yeo SH, Herde MK, Herbison AE
Journal of neuroendocrinology (2021) 339: e13021. 147 318 IHC; tested species: mouse

Different dendritic domains of the GnRH neuron underlie the pulse and surge modes of GnRH secretion in female mice.
Wang L, Guo W, Shen X, Yeo S, Long H, Wang Z, Lyu Q, Herbison AE, Kuang Y
eLife (2020) 9: . 147 318 IHC; tested species: mouse

Identification of a Spinal Circuit for Mechanical and Persistent Spontaneous Itch.
Pan H, Fatima M, Li A, Lee H, Cai W, Horwitz L, Hor CC, Zaher N, Cin M, Slade H, Huang T, et al.
Neuron (2019) 1036: 1135-1149.e6. 147 318 IHC; tested species: mouse

Cat. No.: 147 318

Amount: 200 µl

Price: $425.00

Distinct mechanisms control the specific synaptic functions of Neuroligin 1 and Neuroligin 2.
Wang J, Sudhof T, Wernig M
EMBO reports (2025) : . 147 318 ICC; tested species: mouse

A thalamic-primary auditory cortex circuit mediates resilience to stress.
Li HY, Zhu MZ, Yuan XR, Guo ZX, Pan YD, Li YQ, Zhu XH
Cell (2023) 1867: 1352-1368.e18. 147 318 IHC; tested species: mouse

ICC

IHC

IHC-Fr

Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

1 ) Hermann A et al., Biochem. Biophys. Res. Commun. (2001)
2 ) Kirsch J et al., Brain Res. (1993)
3 ) Kirsch J et al., Eur. J. Neurosci. (1993)

Protocols

ICC

Fixed Cells

IHC

IHC-Fr

Other products for this target

Gephyrin antibody - 147 318 K.O.

References for Gephyrin - 147 318