Tailor-made Antibodies
and Tools for Life Science

Gephyrin antibody - 147 318 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Guinea pig monoclonal recombinant IgG supernatant
Cat. No.: 147 318
Amount: 200 µl
Price: $425.00
Cat. No. 147 318 200 µl purified recombinant IgG supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 200 gallery  
IHC: 1 : 200 (see remarks) gallery  
IHC-P: not recommended
IHC-Fr: 1 : 200 up to 1 : 500 (see remarks) gallery  
Clone GpmAb7a
Subtype IgG2 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks

This antibody is a chimeric antibody based on the well known clone mAb7a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain and has been expressed in mammalian cells.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended. Signal intensities as follows: PFA = acetone > acetone-methanol > methanol.

Data sheet 147_318.pdf

References for Gephyrin - 147 318

Distinct mechanisms control the specific synaptic functions of Neuroligin 1 and Neuroligin 2.
Wang J, Sudhof T, Wernig M
EMBO reports (2025) : . 147 318 ICC; tested species: mouse
The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 147 318 ICC; tested species: mouse
Cnksr2 Loss in Mice Leads to Increased Neural Activity and Behavioral Phenotypes of Epilepsy-Aphasia Syndrome.
Erata E, Gao Y, Purkey AM, Soderblom EJ, McNamara JO, Soderling SH
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4146: 9633-9649. 147 318 ICC; tested species: mouse
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 147 318 ICC; tested species: mouse
Cat. No.: 147 318
Amount: 200 µl
Price: $425.00