Home|||||||Technical Support|

Gephyrin - 147 318

A protein associated with inhibitory neurotransmitter receptors
Monoclonal Recombinant Guinea pig hybridoma supernatant
Cat. No.: 147 318
Amount: 200 µl
Price: 415.00 €
Cat. No. 147 318 200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 200 gallery  
IHC: 1 : 200 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone GpmAb7a
Subtype IgG2 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.
The antibody has been expressed in mammalian cells.

WB: Clone 3B11 (cat. no. 147 111) is highly recommended.

IP: Clone 3B11 (cat. no. 147 111) highly recommended.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Data sheet 147_318.pdf

References for Gephyrin - 147 318

Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 147 318 ICC; tested species: mouse
Identification of a Spinal Circuit for Mechanical and Persistent Spontaneous Itch.
Pan H, Fatima M, Li A, Lee H, Cai W, Horwitz L, Hor CC, Zaher N, Cin M, Slade H, Huang T, et al.
Neuron (2019) 1036: 1135-1149.e6. 147 318 IHC; tested species: mouse
Cat. No.: 147 318
Quantity: 200 µl
Price: 415.00 €
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 147 318 ICC; tested species: mouse
Identification of a Spinal Circuit for Mechanical and Persistent Spontaneous Itch.
Pan H, Fatima M, Li A, Lee H, Cai W, Horwitz L, Hor CC, Zaher N, Cin M, Slade H, Huang T, et al.
Neuron (2019) 1036: 1135-1149.e6. 147 318 IHC; tested species: mouse
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.