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Home > Products > 147 318

Gephyrin antibody - 147 318 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Guinea pig monoclonal recombinant IgG supernatant
Cat. No.: 147 318
Amount: 200 µl
Price: $420.00
Cat. No. 147 318 200 µl purified recombinant IgG supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 200 gallery  
IHC: 1 : 200 (see remarks) gallery  
IHC-P: not recommended
IHC-Fr: 1 : 200 (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone GpmAb7a
Subtype IgG2 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks

This antibody is a chimeric antibody based on the well known clone mAb7a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain and has been expressed in mammalian cells.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
IHC-Fr: PFA fixation is recommended.
Data sheet 147_318.pdf

References for Gephyrin - 147 318

Cnksr2 Loss in Mice Leads to Increased Neural Activity and Behavioral Phenotypes of Epilepsy-Aphasia Syndrome.
Erata E, Gao Y, Purkey AM, Soderblom EJ, McNamara JO, Soderling SH
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4146: 9633-9649. 147 318 ICC; tested species: mouse
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 147 318 ICC; tested species: mouse
Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.
Fang W, Chen X, He J
Neurobiology of stress (2024) 33: 100680. 147 318 IHC; tested species: mouse
Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice.
Brito DVC, Kupke J, Sokolov R, Cambridge S, Both M, Bengtson CP, Rozov A, Oliveira AMM
Molecular psychiatry (2024) : . 147 318 IHC; tested species: mouse
Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 147 318 IHC; tested species: rat
Neuronal activation of Gαq EGL-30/GNAQ late in life rejuvenates cognition across species.
Stevenson ME, Bieri G, Kaletsky R, St Ange J, Remesal L, Pratt KJB, Zhou S, Weng Y, Murphy CT, Villeda SA
Cell reports (2023) 429: 113151. 147 318 IHC; tested species: mouse
A thalamic-primary auditory cortex circuit mediates resilience to stress.
Li HY, Zhu MZ, Yuan XR, Guo ZX, Pan YD, Li YQ, Zhu XH
Cell (2023) 1867: 1352-1368.e18. 147 318 IHC; tested species: mouse
Cx3Cr1-Cre induction leads to microglial activation and IFN-1 signaling caused by DNA damage in early postnatal brain.
Sahasrabuddhe V, Ghosh HS
Cell reports (2022) 383: 110252. 147 318 IHC; tested species: mouse
Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 147 318 IHC; tested species: mouse
Neurotransmitter phenotype switching by spinal excitatory interneurons regulates locomotor recovery after spinal cord injury.
Bertels H, Vicente-Ortiz G, El Kanbi K, Takeoka A
Nature neuroscience (2022) 255: 617-629. 147 318 IHC; tested species: mouse
Gabrb3 is required for the functional integration of pyramidal neuron subtypes in the somatosensory cortex.
Babij R, Ferrer C, Donatelle A, Wacks S, Buch AM, Niemeyer JE, Ma H, Duan ZRS, Fetcho RN, Che A, Otsuka T, et al.
Neuron (2022) : . 147 318 IHC; tested species: mouse
Morphological assessment of GABA and glutamate inputs to GnRH neurons in intact female mice using expansion microscopy.
Yeo SH, Herde MK, Herbison AE
Journal of neuroendocrinology (2021) 339: e13021. 147 318 IHC; tested species: mouse
4E-BP2-dependent translation in cerebellar Purkinje cells controls spatial memory but not autism-like behaviors.
Hooshmandi M, Truong VT, Fields E, Thomas RE, Wong C, Sharma V, Gantois I, Soriano Roque P, Chalkiadaki K, Wu N, Chakraborty A, et al.
Cell reports (2021) 354: 109036. 147 318 IHC; tested species: mouse
Different dendritic domains of the GnRH neuron underlie the pulse and surge modes of GnRH secretion in female mice.
Wang L, Guo W, Shen X, Yeo S, Long H, Wang Z, Lyu Q, Herbison AE, Kuang Y
eLife (2020) 9: . 147 318 IHC; tested species: mouse
Identification of a Spinal Circuit for Mechanical and Persistent Spontaneous Itch.
Pan H, Fatima M, Li A, Lee H, Cai W, Horwitz L, Hor CC, Zaher N, Cin M, Slade H, Huang T, et al.
Neuron (2019) 1036: 1135-1149.e6. 147 318 IHC; tested species: mouse
Cat. No.: 147 318
Amount: 200 µl
Price: $420.00
Cnksr2 Loss in Mice Leads to Increased Neural Activity and Behavioral Phenotypes of Epilepsy-Aphasia Syndrome.
Erata E, Gao Y, Purkey AM, Soderblom EJ, McNamara JO, Soderling SH
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4146: 9633-9649. 147 318 ICC; tested species: mouse
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 147 318 ICC; tested species: mouse
Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.
Fang W, Chen X, He J
Neurobiology of stress (2024) 33: 100680. 147 318 IHC; tested species: mouse
Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice.
Brito DVC, Kupke J, Sokolov R, Cambridge S, Both M, Bengtson CP, Rozov A, Oliveira AMM
Molecular psychiatry (2024) : . 147 318 IHC; tested species: mouse
Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience (2023) 2611: 108306. 147 318 IHC; tested species: rat
Neuronal activation of Gαq EGL-30/GNAQ late in life rejuvenates cognition across species.
Stevenson ME, Bieri G, Kaletsky R, St Ange J, Remesal L, Pratt KJB, Zhou S, Weng Y, Murphy CT, Villeda SA
Cell reports (2023) 429: 113151. 147 318 IHC; tested species: mouse
A thalamic-primary auditory cortex circuit mediates resilience to stress.
Li HY, Zhu MZ, Yuan XR, Guo ZX, Pan YD, Li YQ, Zhu XH
Cell (2023) 1867: 1352-1368.e18. 147 318 IHC; tested species: mouse
Cx3Cr1-Cre induction leads to microglial activation and IFN-1 signaling caused by DNA damage in early postnatal brain.
Sahasrabuddhe V, Ghosh HS
Cell reports (2022) 383: 110252. 147 318 IHC; tested species: mouse
Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 147 318 IHC; tested species: mouse
Neurotransmitter phenotype switching by spinal excitatory interneurons regulates locomotor recovery after spinal cord injury.
Bertels H, Vicente-Ortiz G, El Kanbi K, Takeoka A
Nature neuroscience (2022) 255: 617-629. 147 318 IHC; tested species: mouse
Gabrb3 is required for the functional integration of pyramidal neuron subtypes in the somatosensory cortex.
Babij R, Ferrer C, Donatelle A, Wacks S, Buch AM, Niemeyer JE, Ma H, Duan ZRS, Fetcho RN, Che A, Otsuka T, et al.
Neuron (2022) : . 147 318 IHC; tested species: mouse
Morphological assessment of GABA and glutamate inputs to GnRH neurons in intact female mice using expansion microscopy.
Yeo SH, Herde MK, Herbison AE
Journal of neuroendocrinology (2021) 339: e13021. 147 318 IHC; tested species: mouse
4E-BP2-dependent translation in cerebellar Purkinje cells controls spatial memory but not autism-like behaviors.
Hooshmandi M, Truong VT, Fields E, Thomas RE, Wong C, Sharma V, Gantois I, Soriano Roque P, Chalkiadaki K, Wu N, Chakraborty A, et al.
Cell reports (2021) 354: 109036. 147 318 IHC; tested species: mouse
Different dendritic domains of the GnRH neuron underlie the pulse and surge modes of GnRH secretion in female mice.
Wang L, Guo W, Shen X, Yeo S, Long H, Wang Z, Lyu Q, Herbison AE, Kuang Y
eLife (2020) 9: . 147 318 IHC; tested species: mouse
Identification of a Spinal Circuit for Mechanical and Persistent Spontaneous Itch.
Pan H, Fatima M, Li A, Lee H, Cai W, Horwitz L, Hor CC, Zaher N, Cin M, Slade H, Huang T, et al.
Neuron (2019) 1036: 1135-1149.e6. 147 318 IHC; tested species: mouse
ICC
IHC
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.
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