Tailor-made Antibodies
and Tools for Life Science

Gephyrin antibody - 147 018 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Rabbit monoclonal recombinant IgG supernatant
Cat. No.: 147 018
Amount: 200 µl
Price: $420.00
Cat. No. 147 018 200 µl purified recombinant IgG supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: not recommended
IHC-Fr: 1 : 500 (see remarks) gallery  
Clone RbmAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.
IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended. Signal intensities as follows: acetone > PFA > acetone-methanol > methanol.

Data sheet 147_018.pdf

References for Gephyrin - 147 018

ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 147 018 WB, ICC; tested species: mouse
Cat. No.: 147 018
Amount: 200 µl
Price: $420.00