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Gephyrin - 147 108

A protein associated with inhibitory neurotransmitter receptors
Monoclonal Recombinant human purified IgG
Cat. No.: 147 108
Amount: 50 µg
Price: $415.00
Cat. No. 147 108 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone HumAb7a
Subtype IgG (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with human specific sequences. The antibody can, therefore, be used with standard anti-human secondary reagents.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively, antigen retrieval (10 mM citrate, pH 6.0, overnight at 60°C) can be applied.

Data sheet 147_108.pdf
Cat. No.: 147 108
Quantity: 50 µg
Price: $415.00
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.