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Home > Products > 147 108

Gephyrin antibody - 147 108 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Human monoclonal recombinant IgG
Cat. No.: 147 108
Amount: 50 µg
Price: $420.00
Cat. No. 147 108 50 µg purified recombinant IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not recommended
Clone HumAb7a
Subtype IgG (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with human specific sequences. The antibody can, therefore, be used with standard anti-human secondary reagents.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively, antigen retrieval (10 mM citrate, pH 6.0, overnight at 60°C) can be applied.
Data sheet 147_108.pdf
Cat. No.: 147 108
Amount: 50 µg
Price: $420.00
ICC
IHC
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