Synaptic Systems

Cat. No.: 197 003DY5

Amount: 50 µg

Price: $480.00

ICC

IHC

Background

The essential micronutrient zinc plays an important role in many biological processes like growth, development and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn²⁺ bound water is involved in catalytic reactions. As a charged ion Zn²⁺ cannot cross biological membranes by simple diffusion and must be transported into or out of cells by specialized transport mechanisms. Four Zn transporter proteins, ZnT 1 to ZnT 4, have been cloned. All of them contain several transmembrane domains and a histidine rich intracellular loop.
In the central nervous system Zn plays important roles in synaptic function and plasticity. At synapses Zn is stored in synaptic vesicles by a mechanism depending on the integral membrane protein ZnT 3.

1 ) Salazar G et al., J. Cell. Sci. (2005)
2 ) Salazar G et al., Mol. Biol. Cell (2004)
3 ) Lee JY et al., J. Neurosci. (2000)
4 ) Cole TB et al., Proc. Natl. Acad. Sci. U.S.A. (1999)
5 ) Wenzel HJ et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

Protocols

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 197 003DY5	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with DY 649P. For fluorescence labeled antibodies and other products conjugated to Dyomics^® dyes from Dyomics GmbH the established established fluorescence detection systems can be used. DY-488: λ_ex 493 nm / λ_em 518 nm DY-654: λ_ex 653 nm / λ_em 677 nm DY-649P: λ_ex 654 nm / λ_em 672 nm This product or portions thereof is manufactured under license from Dyomics GmbH. Dyomics is a trademarks of Dyomics GmbH, Jena/Germany. Purchase of reagents related to the Dyomics technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Dyomics. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	DY 649P
Immunogen	Recombinant protein corresponding to AA 2 to 75 from mouse ZnT3 (UniProt Id: P97441)
Reactivity	Reacts with: human (Q99726), rat (Q6QIX3), mouse (P97441). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	197-0P
Data sheet	197_003dy5.pdf

ZnT3 antibody - 197 003DY5