Synaptic Systems

Cat. No.: 166 103

Amount: 50 µg

Price: $375.00

ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 166 103 WB; KD verified; tested species: mouse

WB

ICC

Background

The essential micronutrient Zn plays an important role in many biological processes like growth, development and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn²⁺ bound water is involved in catalytic reactions. As a charged ion Zn²⁺ cannot cross biological membranes by simple diffusion and must be transported by specialized transport mechanisms.
Four Zn transporter proteins ZnT 1, 2, 3 and 4 have been cloned. All of them contain several transmembrane domains and a histidine rich intracellular loop.
ZnT 1 is abundantly expressed and has been suggested to be responsible for the efflux of Zn²⁺ from cells. It is upregulated by high oral Zn doses.

1 ) Ohana E et al., J. Mol. Med. (2006)
2 ) Lovell MA et al., Neurotox Res (2005)
3 ) Nolte C et al., Glia (2004)
4 ) Ford D et al., Proc Nutr Soc (2004)
5 ) Nitzan YB et al., Brain Res. Dev. Brain Res. (2002)
6 ) McMahon RJ et al., Proc. Natl. Acad. Sci. U.S.A. (1998)

Protocols

WB

Cat. No. 166 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 494 to 507 from rat ZnT1 (UniProt Id: Q62720)
Reactivity	Reacts with: rat (Q62720), mouse (Q60738). Other species not tested yet.
Specificity	K.D. validated PubMed: 34871934
Data sheet	166_103.pdf

ZnT1 antibody - 166 103 K.D.

References for ZnT1 - 166 103