Synaptic Systems

Cat. No.: 166 103

Amount: 50 µg

Price: $380.00

Cat. No. 166 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 1000 gallery
Immunogen	Synthetic peptide corresponding to AA 494 to 507 from rat ZnT1 (UniProt Id: Q62720)
Reactivity	Reacts with: rat (Q62720), mouse (Q60738). Other species not tested yet.
Specificity	K.D. validated PubMed: 34871934
Data sheet	166_103.pdf

References for ZnT1 - 166 103

WB (2)

Modulation of ZnT-1 by Let7a unveils a therapeutic potential in amyotrophic lateral sclerosis.
Anzilotti S, Franco C, Valsecchi V, Cuomo O, Lombardi G, Di Muraglia N, De Iesu N, Laudati G, Annunziato L, Canzoniero LMT, Giuseppe P, et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2025) : e00571. 166 103 WB; KD verified; tested species: mouse

ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 166 103 WB; KD verified; tested species: mouse

Cat. No.: 166 103

Amount: 50 µg

Price: $380.00

ICC

IHC-P

Background

The essential micronutrient zinc (Zn²⁺) plays an important role in many biological processes like growth, development, and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn²⁺ bound water is involved in catalytic reactions. As a charged ion Zn²⁺ cannot cross biological membranes by simple diffusion and must be transported by specialized transport mechanisms. Two families of Zn²⁺ transporters, SLC30 (ZnT, Zn²⁺ transporter) and SLC39 (ZIP, Zinc (Zn²⁺)-Iron (Fe²⁺) permease), function in opposite directions to maintain cellular Zn²⁺ homeostasis (1).
Ten Zn²⁺ transporter proteins ZnT1-10 have been described. All of them contain several transmembrane domains and a histidine rich intracellular loop (2).
ZnT1 is abundantly expressed and has been suggested to be responsible for the efflux of Zn²⁺ from cells. It is upregulated by high oral Zn²⁺ doses. ZnT1 plays an essential role in maintaining neuronal Zn²⁺ balance, which is critical for synaptic signalling, neuroprotection, and the prevention of Zn²⁺ toxicity. Dysregulation of ZnT1 in neural tissues has been linked to neurodegenerative conditions and cognitive impairments (3). ZnT1 is involved in regulating Zn²⁺ levels within β-cells, which is essential for insulin storage and secretion. Proper ZnT1 function ensures optimal pancreatic endocrine activity and supports glucose homeostasis (4).

1 ) Huang L et al., Mol Aspects Med (2013)
2 ) Liuzzi JP et al., Annu Rev Nutr (2004)
3 ) Baltaci AK et al., Neurochem Res (2018)
4 ) Zhong ML et al., Histochem Cell Biol (2012)

Protocols

ZnT1 antibody - 166 103 K.D.

References for ZnT1 - 166 103