Cat. No.: 197-0P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 197-0P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 2 to 75 from mouse ZnT3 (UniProt Id: P97441) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 197 002, 197 003, 197 003C3, 197 004, 197 011, 197 011BT, 197 006, 197 003DY2, 197 003DY5 |
| Remarks |
This control protein consists of the recombinant protein (aa 2 - 75 of mouse ZnT 3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 197-0p.pdf |
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The essential micronutrient zinc plays an important role in many biological processes like growth, development and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn2+ bound water is involved in catalytic reactions. As a charged ion Zn2+ cannot cross biological membranes by simple diffusion and must be transported into or out of cells by specialized transport mechanisms. Four Zn transporter proteins, ZnT 1 to ZnT 4, have been cloned. All of them contain several transmembrane domains and a histidine rich intracellular loop.
In the central nervous system Zn plays important roles in synaptic function and plasticity. At synapses Zn is stored in synaptic vesicles by a mechanism depending on the integral membrane protein ZnT 3.