Synaptic Systems

Cat. No.: 197 011BT

Amount: 100 µg

Price: $465.00

WB

IHC

IHC-P

Background

The essential micronutrient zinc plays an important role in many biological processes like growth, development and reproduction. It is found in the active site of many enzymes, where ionization, polarization or replacement of Zn²⁺ bound water is involved in catalytic reactions. As a charged ion Zn²⁺ cannot cross biological membranes by simple diffusion and must be transported into or out of cells by specialized transport mechanisms. Four Zn transporter proteins, ZnT 1 to ZnT 4, have been cloned. All of them contain several transmembrane domains and a histidine rich intracellular loop.
In the central nervous system Zn plays important roles in synaptic function and plasticity. At synapses Zn is stored in synaptic vesicles by a mechanism depending on the integral membrane protein ZnT 3.

1 ) Salazar G et al., J. Cell. Sci. (2005)
2 ) Salazar G et al., Mol. Biol. Cell (2004)
3 ) Lee JY et al., J. Neurosci. (2000)
4 ) Cole TB et al., Proc. Natl. Acad. Sci. U.S.A. (1999)
5 ) Wenzel HJ et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 197 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 IHC: 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	180C1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 2 to 75 from mouse ZnT3 (UniProt Id: P97441)
Reactivity	Reacts with: rat (Q6QIX3), mouse (P97441). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	197-0P
Data sheet	197_011bt.pdf

ZnT3 antibody - 197 011BT