Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 324 002

WRB antibody - 324 002 K.D.

WRB is a receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins
Rabbit polyclonal antiserum
Cat. No.: 324 002
Amount: 200 µl
Price: $355.00
Cat. No. 324 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 40 to 94 from human WRB (UniProt Id: O00258)
Reactivity Reacts with: human (O00258), rat (Q6P6S5), mouse (Q8K0D7).
Other species not tested yet.
Specificity K.D. validated PubMed: 31182645
Data sheet 324_002.pdf

References for WRB - 324 002

The GET insertase exhibits conformational plasticity and induces membrane thinning.
McDowell MA, Heimes M, Enkavi G, Farkas Á, Saar D, Wild K, Schwappach B, Vattulainen I, Sinning I
Nature communications (2023) 141: 7355. 324 002 WB
Differential Modes of Orphan Subunit Recognition for the WRB/CAML Complex.
Inglis AJ, Page KR, Guna A, Voorhees RM
Cell reports (2020) 3011: 3691-3698.e5. 324 002 WB; tested species: human
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 324 002 WB; KD verified; tested species: human
Cat. No.: 324 002
Amount: 200 µl
Price: $355.00
The GET insertase exhibits conformational plasticity and induces membrane thinning.
McDowell MA, Heimes M, Enkavi G, Farkas Á, Saar D, Wild K, Schwappach B, Vattulainen I, Sinning I
Nature communications (2023) 141: 7355. 324 002 WB
Differential Modes of Orphan Subunit Recognition for the WRB/CAML Complex.
Inglis AJ, Page KR, Guna A, Voorhees RM
Cell reports (2020) 3011: 3691-3698.e5. 324 002 WB; tested species: human
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 324 002 WB; KD verified; tested species: human
WB
Background
The widely expressed tryptophan (W)-rich basic protein (WRB), also known as congenital heart disease protein 5 (CHD 5) has initially been described as a nuclear protein that plays a potential role in the pathogenesis of CHD in down syndrome patients.
Recently this protein has been identified as the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.
Other products for this target