Synaptic Systems

Cat. No.: 138 313

Amount: 50 µg

Price: $375.00

Cat. No. 138 313	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 20 from mouse VMaT2 (UniProt Id: Q8BRU6)
Reactivity	Reacts with: rat (Q8BRU6), mouse (Q8BRU6). Other species not tested yet.
Data sheet	138_313.pdf

References for VMaT2 - 138 313

Fluoxetine reverses early-life stress-induced depressive-like behaviors and region-specific alterations of monoamine transporters in female mice.
Zheng JY, Li XX, Liu X, Zhang CC, Sun YX, Ma YN, Wang HL, Su YA, Si TM, Li JT
Pharmacology, biochemistry, and behavior (2024) 237: 173722. 138 313 IHC; tested species: mouse

Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.
Liu X, Sun YX, Zhang CC, Zhang XQ, Zhang Y, Wang T, Ma YN, Wang H, Su YA, Li JT, Si TM, et al.
Neuropharmacology (2021) : 108468. 138 313 IHC; tested species: mouse

Innervation and Neuronal Control of the Mammalian Sinoatrial Node a Comprehensive Atlas.
Hanna P, Dacey MJ, Brennan J, Moss A, Robbins S, Achanta S, Biscola NP, Swid MA, Rajendran PS, Mori S, Hadaya JE, et al.
Circulation research (2021) 1289: 1279-1296. 138 313 IHC; tested species: pig

Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells.
Shwartz Y, Gonzalez-Celeiro M, Chen CL, Pasolli HA, Sheu SH, Fan SM, Shamsi F, Assaad S, Lin ET, Zhang B, Tsai PC, et al.
Cell (2020) : . 138 313 IHC; tested species: mouse

Immunohistochemical analysis of the mouse celiac ganglion: An integrative relay station of the peripheral nervous system.
Kaestner CL, Smith EH, Peirce SG, Hoover DB
The Journal of comparative neurology (2019) : . 138 313 IHC; tested species: mouse

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 138 313 UPTAKE; tested species: rat

Cat. No.: 138 313

Amount: 50 µg

Price: $375.00

IHC

Background

Vesicular monoamine transporters VMaTs mediate the translocation of monoamines (serotonin, histamine, dopamine) from the cytoplasm into secretory vesicles by using a proton electrochemical gradient.
VMaTs are integral membrane proteins with 12 putative trans-membrane domains predicted by sequence analysis. Both, the N- and C-terminus of the proteins are located on the cytoplasmic side.
Two VMaT isoforms, VMaT 1 and VMaT 2, have been described. It has been proposed that VMaT 1 transports monoamines into large dense core vesicles (LDCVs), whereas VMaT 2 is needed for the loading of small synaptic vesicles (SSVs).
In rat VMaT 1 is expressed in the adrenal gland, while VMaT 2 is expressed in brain.

1 ) Jakobsen AM et al., J. Pathol. (2001)
2 ) Colliver TL et al., J. Neurosci. (2000)
3 ) Nirenberg MJ et al., Synapse (1997)
4 ) Nirenberg MJ et al., Proc. Natl. Acad. Sci. U.S.A. (1995)
5 ) Liu Y et al., J. Cell Biol. (1994)
6 ) Höltje M et al., J. Neurosci. (2000)

Protocols

IHC

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VMaT2 antibody - 138 313

References for VMaT2 - 138 313