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VGLUT3 protein - 135-2P

VGluT3 is a synaptic vesicle glutamate transporter
control protein
Cat. No.: 135-2P
Amount: 100 µg
Price: $105.00
Cat. No. 135-2P 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 135 203, 135 204
Remarks

This control protein consists of the antigen (aa 543 - 601 of mouse VGLUT 3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 135-2p.pdf
Cat. No.: 135-2P
Amount: 100 µg
Price: $105.00
Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.

Protocols