VGLUT3 protein - 135-2P

VGluT3 is a synaptic vesicle glutamate transporter

control protein

Cat. No.: 135-2P

Amount: 100 µg

Price: $105.00

Cat. No. 135-2P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	135 203, 135 204
Remarks	This control protein consists of the antigen (aa 543 - 601 of mouse VGLUT 3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	135-2p.pdf

Cat. No.: 135-2P

Amount: 100 µg

Price: $105.00

Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.

1 ) Landry M et al., J. Comp. Neurol. (2004)
2 ) Haverkamp S et al., J. Comp. Neurol. (2004)
3 ) Harkany T et al., Eur. J. Neurosci. (2003)
4 ) Oliveira AL et al., Synapse (2003)
5 ) Fremeau RT et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
6 ) Schäfer MK et al., J. Biol. Chem. (2002)
7 ) Gras C et al., J. Neurosci. (2002)

Protocols

OTHER

Preadsorbtion

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