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VGLUT 3 antibody - 135 204

VGluT3 is a synaptic vesicle glutamate transporter
Guinea pig polyclonal antiserum
Cat. No.: 135 204
Amount: 100 µl
Price: $350.00
Cat. No. 135 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Reactivity Reacts with: mouse (Q8BFU8), rat (Q7TSF2).
Other species not tested yet.
Specificity Specific for mouse VGLUT 3. K.O. PubMed: 28559797
Matching control protein/peptide 135-2P
Remarks

WB: Due to the low abundance of this protein in the brain, immunoblotting is difficult.

Data sheet 135_204.pdf

References for VGLUT 3 - 135 204

Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, Rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 204 WB, IHC; KO verified; tested species: mouse
A disease-associated mutation in thyroid hormone receptor α1 causes hearing loss and sensory hair cell patterning defects in mice.
Affortit C, Blanc F, Nasr J, Ceccato JC, Markossian S, Guyot R, Puel JL, Flamant F, Wang J
Science signaling (2022) 15738: eabj4583. 135 204 IHC; tested species: mouse
Cabp2-Gene Therapy Restores Inner Hair Cell Calcium Currents and Improves Hearing in a DFNB93 Mouse Model.
Oestreicher D, Picher MM, Rankovic V, Moser T, Pangrsic T
Frontiers in molecular neuroscience (2021) 14: 689415. 135 204 IHC; tested species: mouse
VGLUT3-p.A211V variant fuses stereocilia bundles and elongates synaptic ribbons.
Joshi Y, Petit CP, Miot S, Guillet M, Sendin G, Bourien J, Wang J, Pujol R, Mestikawy SE, Puel JL, Nouvian R, et al.
The Journal of physiology (2021) : . 135 204 IHC; tested species: mouse
Otoferlin Is Required for Proper Synapse Maturation and for Maintenance of Inner and Outer Hair Cells in Mouse Models for DFNB9.
Stalmann U, Franke AJ, Al-Moyed H, Strenzke N, Reisinger E
Frontiers in cellular neuroscience (2021) 15: 677543. 135 204 IHC; tested species: mouse
Neurochemically and Hodologically Distinct Ascending VGLUT3 versus Serotonin Subsystems Comprise the r2-Pet1 Median Raphe.
Senft RA, Freret ME, Sturrock N, Dymecki SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4112: 2581-2600. 135 204 IHC; tested species: mouse
Impulse Noise Induced Hidden Hearing Loss, Hair Cell Ciliary Changes and Oxidative Stress in Mice.
Gratias P, Nasr J, Affortit C, Ceccato JC, François F, Casas F, Pujol R, Pucheu S, Puel JL, Wang J
Antioxidants (Basel, Switzerland) (2021) 1012: . 135 204 IHC; tested species: mouse
Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina.
Lux UT, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH
International journal of molecular sciences (2021) 2215: . 135 204 IHC; tested species: mouse
Perinatal interference with the serotonergic system affects VTA function in the adult via glutamate co-transmission.
Cunha C, Smiley JF, Chuhma N, Shah R, Bleiwas C, Menezes EC, Seal RP, Edwards RH, Rayport S, Ansorge MS, Castellanos FX, et al.
Molecular psychiatry (2020) : . 135 204 IHC; tested species: mouse
A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons.
Okaty BW, Sturrock N, Escobedo Lozoya Y, Chang Y, Senft RA, Lyon KA, Alekseyenko OV, Dymecki SM
eLife (2020) 9: . 135 204 IHC; tested species: mouse
Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 135 204 IHC; tested species: mouse
Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae.
Jiang D, Burger CA, Casasent AK, Albrecht NE, Li F, Samuel MA
The Journal of comparative neurology (2019) : . 135 204 IHC; tested species: mouse
Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse.
Tulloch AJ, Teo S, Carvajal BV, Tessier-Lavigne M, Jaworski A
The Journal of comparative neurology (2019) 52718: 2948-2972. 135 204 IHC; tested species: mouse
Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets.
Sonntag M, Blosa M, Schmidt S, Reimann K, Blum K, Eckrich T, Seeger G, Hecker D, Schick B, Arendt T, Engel J, et al.
BMC biology (2018) 161: 99. 135 204 IHC; tested species: mouse
Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, Rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 204 WB, IHC; KO verified; tested species: mouse
Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus.
Heise C, Schroeder JC, Schoen M, Halbedl S, Reim D, Woelfle S, Kreutz MR, Schmeisser MJ, Boeckers TM
Frontiers in cellular neuroscience (2016) 10: 106. 135 204 IHC
Mice deficient of glutamatergic signaling from intrinsically photosensitive retinal ganglion cells exhibit abnormal circadian photoentrainment.
Purrier N, Engeland WC, Kofuji P
PloS one (2014) 910: e111449. 135 204 IHC; tested species: mouse
Cat. No.: 135 204
Amount: 100 µl
Price: $350.00
Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, Rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 204 WB, IHC; KO verified; tested species: mouse
A disease-associated mutation in thyroid hormone receptor α1 causes hearing loss and sensory hair cell patterning defects in mice.
Affortit C, Blanc F, Nasr J, Ceccato JC, Markossian S, Guyot R, Puel JL, Flamant F, Wang J
Science signaling (2022) 15738: eabj4583. 135 204 IHC; tested species: mouse
Cabp2-Gene Therapy Restores Inner Hair Cell Calcium Currents and Improves Hearing in a DFNB93 Mouse Model.
Oestreicher D, Picher MM, Rankovic V, Moser T, Pangrsic T
Frontiers in molecular neuroscience (2021) 14: 689415. 135 204 IHC; tested species: mouse
VGLUT3-p.A211V variant fuses stereocilia bundles and elongates synaptic ribbons.
Joshi Y, Petit CP, Miot S, Guillet M, Sendin G, Bourien J, Wang J, Pujol R, Mestikawy SE, Puel JL, Nouvian R, et al.
The Journal of physiology (2021) : . 135 204 IHC; tested species: mouse
Otoferlin Is Required for Proper Synapse Maturation and for Maintenance of Inner and Outer Hair Cells in Mouse Models for DFNB9.
Stalmann U, Franke AJ, Al-Moyed H, Strenzke N, Reisinger E
Frontiers in cellular neuroscience (2021) 15: 677543. 135 204 IHC; tested species: mouse
Neurochemically and Hodologically Distinct Ascending VGLUT3 versus Serotonin Subsystems Comprise the r2-Pet1 Median Raphe.
Senft RA, Freret ME, Sturrock N, Dymecki SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4112: 2581-2600. 135 204 IHC; tested species: mouse
Impulse Noise Induced Hidden Hearing Loss, Hair Cell Ciliary Changes and Oxidative Stress in Mice.
Gratias P, Nasr J, Affortit C, Ceccato JC, François F, Casas F, Pujol R, Pucheu S, Puel JL, Wang J
Antioxidants (Basel, Switzerland) (2021) 1012: . 135 204 IHC; tested species: mouse
Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina.
Lux UT, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH
International journal of molecular sciences (2021) 2215: . 135 204 IHC; tested species: mouse
Perinatal interference with the serotonergic system affects VTA function in the adult via glutamate co-transmission.
Cunha C, Smiley JF, Chuhma N, Shah R, Bleiwas C, Menezes EC, Seal RP, Edwards RH, Rayport S, Ansorge MS, Castellanos FX, et al.
Molecular psychiatry (2020) : . 135 204 IHC; tested species: mouse
A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons.
Okaty BW, Sturrock N, Escobedo Lozoya Y, Chang Y, Senft RA, Lyon KA, Alekseyenko OV, Dymecki SM
eLife (2020) 9: . 135 204 IHC; tested species: mouse
Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 135 204 IHC; tested species: mouse
Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae.
Jiang D, Burger CA, Casasent AK, Albrecht NE, Li F, Samuel MA
The Journal of comparative neurology (2019) : . 135 204 IHC; tested species: mouse
Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3Cre mouse.
Tulloch AJ, Teo S, Carvajal BV, Tessier-Lavigne M, Jaworski A
The Journal of comparative neurology (2019) 52718: 2948-2972. 135 204 IHC; tested species: mouse
Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets.
Sonntag M, Blosa M, Schmidt S, Reimann K, Blum K, Eckrich T, Seeger G, Hecker D, Schick B, Arendt T, Engel J, et al.
BMC biology (2018) 161: 99. 135 204 IHC; tested species: mouse
Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, Rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 204 WB, IHC; KO verified; tested species: mouse
Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus.
Heise C, Schroeder JC, Schoen M, Halbedl S, Reim D, Woelfle S, Kreutz MR, Schmeisser MJ, Boeckers TM
Frontiers in cellular neuroscience (2016) 10: 106. 135 204 IHC
Mice deficient of glutamatergic signaling from intrinsically photosensitive retinal ganglion cells exhibit abnormal circadian photoentrainment.
Purrier N, Engeland WC, Kofuji P
PloS one (2014) 910: e111449. 135 204 IHC; tested species: mouse
Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.