Tailor-made Antibodies
and Tools for Life Science

VGLUT3 antibody - 135 203 K.O.

VGluT3 is a synaptic vesicle glutamate transporter
Rabbit polyclonal purified antibody
Cat. No.: 135 203
Amount: 50 µg
Price: $405.00
Cat. No. 135 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: yes
IHC: 1 : 100 up to 1 : 1000 gallery  
IHC-P: 1 : 200 gallery  
FACS: yes
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Reactivity Reacts with: rat (Q7TSF2), mouse (Q8BFU8).
No signal: human.
Other species not tested yet.
Specificity K.O. validated PubMed: 21297271
Matching control protein/peptide 135-2P
Remarks

WB: Due to the low abundance of this protein in the brain, immunoblotting is difficult.

Data sheet 135_203.pdf

References for VGLUT3 - 135 203

Vesicular Acetylcholine Transporter Alters Cholinergic Tone and Synaptic Plasticity in DYT1 Dystonia.
Tassone A, Martella G, Meringolo M, Vanni V, Sciamanna G, Ponterio G, Imbriani P, Bonsi P, Pisani A
Movement disorders : official journal of the Movement Disorder Society (2021) : . 135 203 WB; tested species: mouse
Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.
Cheret C, Ganzella M, Preobraschenski J, Jahn R, Ahnert-Hilger G
Cell reports (2021) 342: 108623. 135 203 WB, ICC; tested species: human,mouse
Vesicular glutamate transporters play a role in neuronal differentiation of cultured SVZ-derived neural precursor cells.
Sánchez-Mendoza EH, Bellver-Landete V, Arce C, Doeppner TR, Hermann DM, Oset-Gasque MJ
PloS one (2017) 125: e0177069. 135 203 WB, ICC
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 135 203 WB
Lack of evidence for vesicular glutamate transporter expression in mouse astrocytes.
Li D, Hérault K, Silm K, Evrard A, Wojcik S, Oheim M, Herzog E, Ropert N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3310: 4434-55. 135 203 WB, IHC; KO verified; tested species: mouse
Region- and age-specific changes in glutamate transport in the AβPP23 mouse model for Alzheimer's disease.
Schallier A, Smolders I, Van Dam D, Loyens E, De Deyn PP, Michotte A, Michotte Y, Massie A
Journal of Alzheimer's disease : JAD (2011) 242: 287-300. 135 203 WB, IHC; KO verified; tested species: mouse
Loss of system x(c)- does not induce oxidative stress but decreases extracellular glutamate in hippocampus and influences spatial working memory and limbic seizure susceptibility.
De Bundel D, Schallier A, Loyens E, Fernando R, Miyashita H, Van Liefferinge J, Vermoesen K, Bannai S, Sato H, Michotte Y, Smolders I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3115: 5792-803. 135 203 WB; tested species: mouse
Transient focal cerebral ischemia significantly alters not only EAATs but also VGLUTs expression in rats: relevance of changes in reactive astroglia.
Sánchez-Mendoza E, Burguete MC, Castelló-Ruiz M, González MP, Roncero C, Salom JB, Arce C, Cañadas S, Torregrosa G, Alborch E, Oset-Gasque MJ, et al.
Journal of neurochemistry (2010) 1135: 1343-55. 135 203 IHC, WB; tested species: rat
An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size.
Wojcik SM, Rhee JS, Herzog E, Sigler A, Jahn R, Takamori S, Brose N, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2004) 10118: 7158-63. 135 203 WB; tested species: mouse
Cat. No.: 135 203
Amount: 50 µg
Price: $405.00