Synaptic Systems

Cat. No.: 131 103

Amount: 50 µg

Price: $665.00

Cat. No. 131 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 100 up to 1 : 200 (see remarks) gallery IHC: yes IHC-P: not tested yet
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity	Reacts with: human (Q9H598), rat (O35458), mouse (O35633). Other species not tested yet.
Specificity	K.O. validated PubMed: 19052203
Remarks	WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: This antibody is intended for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding to living neurons. Further details see Martens et al. 2008.
Data sheet	131_103.pdf

References for VGAT - 131 103

WB (2)
ICC (6)
IHC (5)
DNA-PAINT (1)
UPTAKE (3)

A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.
Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT
Current research in physiology (2024) 7: 100121. 131 103 WB; tested species: mouse

Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103 WB, ICC, IHC, UPTAKE; KO verified; tested species: mouse,rat

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 131 103 ICC; tested species: rat

Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course.
Esposito A, Falace A, Wagner M, Gal M, Mei D, Conti V, Pisano T, Aprile D, Cerullo MS, De Fusco A, Giovedì S, et al.
Brain : a journal of neurology (2019) : . 131 103 ICC; tested species: mouse

Inhibitory synapse dynamics: coordinated presynaptic and postsynaptic mobility and the major contribution of recycled vesicles to new synapse formation.
Dobie FA, Craig AM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3129: 10481-93. 131 103 ICC

Intrinsic calcium dynamics control botulinum toxin A susceptibility in distinct neuronal populations.
Grumelli C, Corradini I, Matteoli M, Verderio C
Cell calcium (2010) 475: 419-24. 131 103 ICC

VGLUT1 and VGAT are sorted to the same population of synaptic vesicles in subsets of cortical axon terminals.
Fattorini G, Verderio C, Melone M, Giovedì S, Benfenati F, Matteoli M, Conti F
Journal of neurochemistry (2009) 1105: 1538-46. 131 103 ICC

NaV1.1 haploinsufficiency impairs glutamatergic and GABAergic neuron function in the thalamus.
Studtmann C, Ladislav M, Topolski MA, Safari M, Swanger SA
Neurobiology of disease (2022) 167: 105672. 131 103 IHC; tested species: mouse

Excitatory neuronal CHD8 in the regulation of neocortical development and sensory-motor behaviors.
Kweon H, Jung WB, Im GH, Ryoo J, Lee JH, Do H, Choi Y, Song YH, Jung H, Park H, Qiu LR, et al.
Cell reports (2021) 348: 108780. 131 103 IHC; tested species: mouse

Inhibitory control in neuronal networks relies on the extracellular matrix integrity.
Dzyubenko E, Fleischer M, Manrique-Castano D, Borbor M, Kleinschnitz C, Faissner A, Hermann DM
Cellular and molecular life sciences : CMLS (2021) 7814: 5647-5663. 131 103 IHC; tested species: mouse

Nuclei-specific differences in nerve terminal distribution, morphology, and development in mouse visual thalamus.
Hammer S, Carrillo GL, Govindaiah G, Monavarfeshani A, Bircher JS, Su J, Guido W, Fox MA
Neural development (2014) 9: 16. 131 103 IHC

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 131 103 DNA-PAINT; tested species: rat

The potassium channel subunit Kvβ1 serves as a major control point for synaptic facilitation.
Cho IH, Panzera LC, Chin M, Alpizar SA, Olveda GE, Hill RA, Hoppa MB
Proceedings of the National Academy of Sciences of the United States of America (2020) 11747: 29937-29947. 131 103 UPTAKE; tested species: rat

Astrocyte secreted proteins selectively increase hippocampal GABAergic axon length, branching, and synaptogenesis.
Hughes EG, Elmariah SB, Balice-Gordon RJ
Molecular and cellular neurosciences (2010) 431: 136-45. 131 103 UPTAKE; tested species: rat

Cat. No.: 131 103

Amount: 50 µg

Price: $665.00

References
WB (2)
ICC (6)
IHC (5)
DNA-PAINT (1)
UPTAKE (3)

Intrinsic calcium dynamics control botulinum toxin A susceptibility in distinct neuronal populations.
Grumelli C, Corradini I, Matteoli M, Verderio C
Cell calcium (2010) 475: 419-24. 131 103 ICC

ICC

Background

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

1 ) Chaudhry FA et al., J. Neurosci. (1998)
2 ) McIntire SL et al., Nature (1997)
3 ) Sagné C et al., FEBS Lett. (1997)
4 ) Hell JW et al., EMBO J. (1988)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

Other products for this target

VGAT antibody luminal domain - 131 103 K.O.

References for VGAT - 131 103