Synaptic Systems - Veli1/2/3

Cat. No.: 184 002

Amount: 200 µl

Price: $355.00

Cat. No. 184 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 232 from rat Velis1 (UniProt Id: Q9Z250)
Reactivity	Reacts with: human (O14910, Q9HAP6, Q9NUP9), rat (Q9Z250, Q9Z252, Q792I0), mouse (Q8JZS0, O88951, O88952). Other species not tested yet.
Specificity	Recognizes all three isoforms veli 1, 2 and 3.
Matching control protein/peptide	184-0P
Data sheet	184_002.pdf

References for Veli1/2/3 - 184 002

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 184 002 WB; tested species: human

Regulation of Liprin-α phase separation by CASK is disrupted by a mutation in its CaM kinase domain.
Tibbe D, Ferle P, Krisp C, Nampoothiri S, Mirzaa G, Assaf M, Parikh S, Kutsche K, Kreienkamp HJ
Life science alliance (2022) 510: . 184 002 WB; tested species: human

Functional analysis of CASK transcript variants expressed in human brain.
Tibbe D, Pan YE, Reißner C, Harms FL, Kreienkamp HJ
PloS one (2021) 166: e0253223. 184 002 WB; tested species: human

Cat. No.: 184 002

Amount: 200 µl

Price: $355.00

Functional analysis of CASK transcript variants expressed in human brain.
Tibbe D, Pan YE, Reißner C, Harms FL, Kreienkamp HJ
PloS one (2021) 166: e0253223. 184 002 WB; tested species: human

ICC

Background

The complex of the multi-adaptor-proteins CASK, Mint and Veli, also referred to as MALS and Lin 7, is conserved across kingdoms and may be involved in localizing synaptic vesicles at synaptic terminals. Three Veli isoforms (1, 2 and 3) with different expression patterns have been described, so far.
Veli proteins have also been suggested to participate in regulating the recruitment of neurotransmitter receptors to the post synaptic density (PSD) by interaction with their PDZ domains.

1 ) Olsen O et al., Curr. Opin. Cell Biol. (2006)
2 ) Olsen O et al., J. Cell Biol. (2005)
3 ) Joglekar AP et al., J. Biol. Chem. (2003)

Protocols

ICC

OTHER

Preabsorbtion

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Veli1/2/3 antibody - 184 002

References for Veli1/2/3 - 184 002