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Home > Products > 139 105

VAChT antibody - 139 105

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles
Guinea pig polyclonal purified antibody
Cat. No.: 139 105
Amount: 50 µg
Price: $475.00
Cat. No. 139 105 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
iDISCO: 1 : 400
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: rat (Q62666), mouse (O35304), human (Q16572).
Other species not tested yet.
Matching control protein/peptide 139-1P
Remarks

This antibody is less sensitive compared to the rabbit antibody.
WB: VAChT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
Data sheet 139_105.pdf

References for VAChT - 139 105

Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody.
Fabris F, Šoštarić P, Matak I, Binz T, Toffan A, Simonato M, Montecucco C, Pirazzini M, Rossetto O
International journal of molecular sciences (2022) 238: . 139 105 ICC; tested species: rat
Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.
Djemil S, Ressel CR, Abdel-Ghani M, Schneeweis AK, Pak DTS
Cellular and molecular neurobiology (2020) : . 139 105 ICC; tested species: rat
Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 139 105 ICC; tested species: mouse
Neuregulin 1-ErbB module in C-bouton synapses on somatic motor neurons: molecular compartmentation and response to peripheral nerve injury.
Casanovas A, Salvany S, Lahoz V, Tarabal O, Piedrafita L, Sabater R, Hernández S, Calderó J, Esquerda JE
Scientific reports (2017) 7: 40155. 139 105 ICC
Electrophysiology and 3D-imaging reveal properties of human intracardiac neurons and increased excitability with atrial fibrillation.
Ashton JL, Prince B, Sands G, Argent L, Anderson M, Smith JEG, Tedoldi A, Ahmad A, Baddeley D, Pereira AG, Lever N, et al.
The Journal of physiology (2024) : . 139 105 IHC; tested species: human
DLK signaling in axotomized neurons triggers complement activation and loss of upstream synapses.
Asghari Adib E, Shadrach JL, Reilly-Jankowiak L, Dwivedi MK, Rogers AE, Shahzad S, Passino R, Giger RJ, Pierchala BA, Collins CA
Cell reports (2024) 432: 113801. 139 105 IHC; tested species: mouse
Persistent NRG1 Type III Overexpression in Spinal Motor Neurons Has No Therapeutic Effect on ALS-Related Pathology in SOD1G93A Mice.
Hernández S, Salvany S, Casanovas A, Piedrafita L, Soto-Bernardini MC, Tarabal O, Blasco A, Gras S, Gatius A, Schwab MH, Calderó J, et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2023) : . 139 105 IHC; tested species: mouse
Thalamic regulation of ocular dominance plasticity in adult visual cortex.
Qin Y, Ahmadlou M, Suhai S, Neering P, de Kraker L, Heimel JA, Levelt CN
eLife (2023) 12: . 139 105 IHC; tested species: mouse
Facial neuromuscular junctions and brainstem nuclei are the target of tetanus neurotoxin in cephalic tetanus.
Fabris F, Varani S, Tonellato M, Matak I, Šoštarić P, Meglić P, Caleo M, Megighian A, Rossetto O, Montecucco C, Pirazzini M, et al.
JCI insight (2023) 811: . 139 105 IHC; tested species: mouse,rat
Characteristics of Electrical Synapses, C-terminals and Small-conductance Ca2+ activated Potassium Channels in the Sexually Dimorphic Cremaster Motor Nucleus in Spinal Cord of Mouse and Rat.
Singhal P, Senecal JMM, Senecal JEM, Silwal P, Lynn BD, Nagy JI
Neuroscience (2023) 521: 58-76. 139 105 IHC; tested species: mouse
ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control.
Khan MN, Cherukuri P, Negro F, Rajput A, Fabrowski P, Bansal V, Lancelin C, Lee TI, Bian Y, Mayer WP, Akay T, et al.
PLoS biology (2022) 2012: e3001923. 139 105 IHC; tested species: mouse
Endothelial PlexinD1 signaling instructs spinal cord vascularization and motor neuron development.
Vieira JR, Shah B, Dupraz S, Paredes I, Himmels P, Schermann G, Adler H, Motta A, Gärtner L, Navarro-Aragall A, Ioannou E, et al.
Neuron (2022) 11024: 4074-4089.e6. 139 105 IHC; tested species: mouse
Early regeneration of axons following peripheral nerve injury is enhanced if p75NTR is eliminated from the surrounding pathway.
McGregor C, Sabatier M, English A
The European journal of neuroscience (2021) 532: 663-672. 139 105 IHC; tested species: mouse
Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system.
Gras S, Blasco A, Mòdol-Caballero G, Tarabal O, Casanovas A, Piedrafita L, Barranco A, Das T, Rueda R, Pereira SL, Navarro X, et al.
Aging (2021) 1314: 18051-18093. 139 105 IHC; tested species: mouse
C1ql1 is expressed in adult outer hair cells of the cochlea in a tonotopic gradient.
Biswas J, Pijewski RS, Makol R, Miramontes TG, Thompson BL, Kresic LC, Burghard AL, Oliver DL, Martinelli DC
PloS one (2021) 165: e0251412. 139 105 IHC; tested species: mouse
Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury.
Salvany S, Casanovas A, Piedrafita L, Tarabal O, Hernández S, Calderó J, Esquerda JE
Glia (2021) : . 139 105 IHC; tested species: mouse
Mapping of Extrinsic Innervation of the Gastrointestinal Tract in the Mouse Embryo.
Niu X, Liu L, Wang T, Chuan X, Yu Q, Du M, Gu Y, Wang L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4035: 6691-6708. 139 105 IHC; tested species: mouse
Hair cell α9α10 nicotinic acetylcholine receptor functional expression regulated by ligand binding and deafness gene products.
Gu S, Knowland D, Matta JA, O'Carroll ML, Davini WB, Dhara M, Kweon HJ, Bredt DS
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 139 105 IHC; tested species: mouse
Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model.
Etxeberria-Rekalde E, Alzola-Aldamizetxebarria S, Flunkert S, Hable I, Daurer M, Neddens J, Hutter-Paier B
Frontiers in molecular neuroscience (2020) 13: 617229. 139 105 IHC; tested species: mouse
Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.
Blasco A, Gras S, Mòdol-Caballero G, Tarabal O, Casanovas A, Piedrafita L, Barranco A, Das T, Pereira SL, Navarro X, Rueda R, et al.
Journal of cachexia, sarcopenia and muscle (2020) : . 139 105 IHC; tested species: mouse
Chondroitin sulfate proteoglycan-5 forms perisynaptic matrix assemblies in the adult rat cortex.
Pintér A, Hevesi Z, Zahola P, Alpár A, Hanics J
Cellular signalling (2020) 74: 109710. 139 105 IHC; tested species: rat
Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS.
Bączyk M, Alami NO, Delestrée N, Martinot C, Tang L, Commisso B, Bayer D, Doisne N, Frankel W, Manuel M, Roselli F, et al.
The Journal of experimental medicine (2020) 2178: . 139 105 IHC; tested species: mouse
Cholinergic neuroplasticity in asthma driven by TrkB signaling.
Dragunas G, Woest ME, Nijboer S, Bos ST, van Asselt J, de Groot AP, Vohlídalová E, Vermeulen CJ, Ditz B, Vonk JM, Koppelman GH, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020) : . 139 105 IHC; tested species: human
The Val66Met BDNF Polymorphism and Peripheral Nerve Injury: Enhanced Regeneration in Mouse Met-Carriers Is Not Further Improved With Activity-Dependent Treatment.
McGregor CE, Irwin AM, English AW
Neurorehabilitation and neural repair (2019) : 1545968319846131. 139 105 IHC; tested species: mouse
Localization and dynamic changes of neuregulin-1 at C-type synaptic boutons in association with motor neuron injury and repair.
Salvany S, Casanovas A, Tarabal O, Piedrafita L, Hernández S, Santafé M, Soto-Bernardini MC, Calderó J, Schwab MH, Esquerda JE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201802329R. 139 105 IHC; tested species: mouse
Glial Activation and Central Synapse Loss, but Not Motoneuron Degeneration, Are Prevented by the Sigma-1 Receptor Agonist PRE-084 in the Smn2B/- Mouse Model of Spinal Muscular Atrophy.
Cerveró C, Blasco A, Tarabal O, Casanovas A, Piedrafita L, Navarro X, Esquerda JE, Calderó J
Journal of neuropathology and experimental neurology (2018) : . 139 105 IHC; tested species: mouse
Hypoexcitability precedes denervation in the large fast-contracting motor units in two unrelated mouse models of ALS.
Martinez-Silva ML, Imhoff-Manuel RD, Sharma A, Heckman CJ, Shneider NA, Roselli F, Zytnicki D, Manuel M
eLife (2018) 7: . 139 105 IHC; tested species: mouse
Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb.
Case DT, Burton SD, Gedeon JY, Williams SG, Urban NN, Seal RP
Nature communications (2017) 81: 652. 139 105 IHC; tested species: mouse
Nuclei-specific differences in nerve terminal distribution, morphology, and development in mouse visual thalamus.
Hammer S, Carrillo GL, Govindaiah G, Monavarfeshani A, Bircher JS, Su J, Guido W, Fox MA
Neural development (2014) 9: 16. 139 105 IHC
Neuregulin-1 is concentrated in the postsynaptic subsurface cistern of C-bouton inputs to α-motoneurons and altered during motoneuron diseases.
Gallart-Palau X, Tarabal O, Casanovas A, Sábado J, Correa FJ, Hereu M, Piedrafita L, Calderó J, Esquerda JE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014) 288: 3618-32. 139 105 IHC
Altered Dynein Axonemal Assembly Factor 1 Expression in C-Boutons in Bulbar and Spinal Cord Motor-Neurons in Sporadic Amyotrophic Lateral Sclerosis.
Andrés-Benito P, Povedano M, Torres P, Portero-Otín M, Ferrer I
Journal of neuropathology and experimental neurology (2019) : . 139 105 IHC-P; tested species: human
Cat. No.: 139 105
Amount: 50 µg
Price: $475.00
Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody.
Fabris F, Šoštarić P, Matak I, Binz T, Toffan A, Simonato M, Montecucco C, Pirazzini M, Rossetto O
International journal of molecular sciences (2022) 238: . 139 105 ICC; tested species: rat
Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.
Djemil S, Ressel CR, Abdel-Ghani M, Schneeweis AK, Pak DTS
Cellular and molecular neurobiology (2020) : . 139 105 ICC; tested species: rat
Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 139 105 ICC; tested species: mouse
Neuregulin 1-ErbB module in C-bouton synapses on somatic motor neurons: molecular compartmentation and response to peripheral nerve injury.
Casanovas A, Salvany S, Lahoz V, Tarabal O, Piedrafita L, Sabater R, Hernández S, Calderó J, Esquerda JE
Scientific reports (2017) 7: 40155. 139 105 ICC
Electrophysiology and 3D-imaging reveal properties of human intracardiac neurons and increased excitability with atrial fibrillation.
Ashton JL, Prince B, Sands G, Argent L, Anderson M, Smith JEG, Tedoldi A, Ahmad A, Baddeley D, Pereira AG, Lever N, et al.
The Journal of physiology (2024) : . 139 105 IHC; tested species: human
DLK signaling in axotomized neurons triggers complement activation and loss of upstream synapses.
Asghari Adib E, Shadrach JL, Reilly-Jankowiak L, Dwivedi MK, Rogers AE, Shahzad S, Passino R, Giger RJ, Pierchala BA, Collins CA
Cell reports (2024) 432: 113801. 139 105 IHC; tested species: mouse
Persistent NRG1 Type III Overexpression in Spinal Motor Neurons Has No Therapeutic Effect on ALS-Related Pathology in SOD1G93A Mice.
Hernández S, Salvany S, Casanovas A, Piedrafita L, Soto-Bernardini MC, Tarabal O, Blasco A, Gras S, Gatius A, Schwab MH, Calderó J, et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2023) : . 139 105 IHC; tested species: mouse
Thalamic regulation of ocular dominance plasticity in adult visual cortex.
Qin Y, Ahmadlou M, Suhai S, Neering P, de Kraker L, Heimel JA, Levelt CN
eLife (2023) 12: . 139 105 IHC; tested species: mouse
Facial neuromuscular junctions and brainstem nuclei are the target of tetanus neurotoxin in cephalic tetanus.
Fabris F, Varani S, Tonellato M, Matak I, Šoštarić P, Meglić P, Caleo M, Megighian A, Rossetto O, Montecucco C, Pirazzini M, et al.
JCI insight (2023) 811: . 139 105 IHC; tested species: mouse,rat
Characteristics of Electrical Synapses, C-terminals and Small-conductance Ca2+ activated Potassium Channels in the Sexually Dimorphic Cremaster Motor Nucleus in Spinal Cord of Mouse and Rat.
Singhal P, Senecal JMM, Senecal JEM, Silwal P, Lynn BD, Nagy JI
Neuroscience (2023) 521: 58-76. 139 105 IHC; tested species: mouse
ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control.
Khan MN, Cherukuri P, Negro F, Rajput A, Fabrowski P, Bansal V, Lancelin C, Lee TI, Bian Y, Mayer WP, Akay T, et al.
PLoS biology (2022) 2012: e3001923. 139 105 IHC; tested species: mouse
Endothelial PlexinD1 signaling instructs spinal cord vascularization and motor neuron development.
Vieira JR, Shah B, Dupraz S, Paredes I, Himmels P, Schermann G, Adler H, Motta A, Gärtner L, Navarro-Aragall A, Ioannou E, et al.
Neuron (2022) 11024: 4074-4089.e6. 139 105 IHC; tested species: mouse
Early regeneration of axons following peripheral nerve injury is enhanced if p75NTR is eliminated from the surrounding pathway.
McGregor C, Sabatier M, English A
The European journal of neuroscience (2021) 532: 663-672. 139 105 IHC; tested species: mouse
Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system.
Gras S, Blasco A, Mòdol-Caballero G, Tarabal O, Casanovas A, Piedrafita L, Barranco A, Das T, Rueda R, Pereira SL, Navarro X, et al.
Aging (2021) 1314: 18051-18093. 139 105 IHC; tested species: mouse
C1ql1 is expressed in adult outer hair cells of the cochlea in a tonotopic gradient.
Biswas J, Pijewski RS, Makol R, Miramontes TG, Thompson BL, Kresic LC, Burghard AL, Oliver DL, Martinelli DC
PloS one (2021) 165: e0251412. 139 105 IHC; tested species: mouse
Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury.
Salvany S, Casanovas A, Piedrafita L, Tarabal O, Hernández S, Calderó J, Esquerda JE
Glia (2021) : . 139 105 IHC; tested species: mouse
Mapping of Extrinsic Innervation of the Gastrointestinal Tract in the Mouse Embryo.
Niu X, Liu L, Wang T, Chuan X, Yu Q, Du M, Gu Y, Wang L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4035: 6691-6708. 139 105 IHC; tested species: mouse
Hair cell α9α10 nicotinic acetylcholine receptor functional expression regulated by ligand binding and deafness gene products.
Gu S, Knowland D, Matta JA, O'Carroll ML, Davini WB, Dhara M, Kweon HJ, Bredt DS
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 139 105 IHC; tested species: mouse
Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model.
Etxeberria-Rekalde E, Alzola-Aldamizetxebarria S, Flunkert S, Hable I, Daurer M, Neddens J, Hutter-Paier B
Frontiers in molecular neuroscience (2020) 13: 617229. 139 105 IHC; tested species: mouse
Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice.
Blasco A, Gras S, Mòdol-Caballero G, Tarabal O, Casanovas A, Piedrafita L, Barranco A, Das T, Pereira SL, Navarro X, Rueda R, et al.
Journal of cachexia, sarcopenia and muscle (2020) : . 139 105 IHC; tested species: mouse
Chondroitin sulfate proteoglycan-5 forms perisynaptic matrix assemblies in the adult rat cortex.
Pintér A, Hevesi Z, Zahola P, Alpár A, Hanics J
Cellular signalling (2020) 74: 109710. 139 105 IHC; tested species: rat
Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS.
Bączyk M, Alami NO, Delestrée N, Martinot C, Tang L, Commisso B, Bayer D, Doisne N, Frankel W, Manuel M, Roselli F, et al.
The Journal of experimental medicine (2020) 2178: . 139 105 IHC; tested species: mouse
Cholinergic neuroplasticity in asthma driven by TrkB signaling.
Dragunas G, Woest ME, Nijboer S, Bos ST, van Asselt J, de Groot AP, Vohlídalová E, Vermeulen CJ, Ditz B, Vonk JM, Koppelman GH, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020) : . 139 105 IHC; tested species: human
The Val66Met BDNF Polymorphism and Peripheral Nerve Injury: Enhanced Regeneration in Mouse Met-Carriers Is Not Further Improved With Activity-Dependent Treatment.
McGregor CE, Irwin AM, English AW
Neurorehabilitation and neural repair (2019) : 1545968319846131. 139 105 IHC; tested species: mouse
Localization and dynamic changes of neuregulin-1 at C-type synaptic boutons in association with motor neuron injury and repair.
Salvany S, Casanovas A, Tarabal O, Piedrafita L, Hernández S, Santafé M, Soto-Bernardini MC, Calderó J, Schwab MH, Esquerda JE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201802329R. 139 105 IHC; tested species: mouse
Glial Activation and Central Synapse Loss, but Not Motoneuron Degeneration, Are Prevented by the Sigma-1 Receptor Agonist PRE-084 in the Smn2B/- Mouse Model of Spinal Muscular Atrophy.
Cerveró C, Blasco A, Tarabal O, Casanovas A, Piedrafita L, Navarro X, Esquerda JE, Calderó J
Journal of neuropathology and experimental neurology (2018) : . 139 105 IHC; tested species: mouse
Hypoexcitability precedes denervation in the large fast-contracting motor units in two unrelated mouse models of ALS.
Martinez-Silva ML, Imhoff-Manuel RD, Sharma A, Heckman CJ, Shneider NA, Roselli F, Zytnicki D, Manuel M
eLife (2018) 7: . 139 105 IHC; tested species: mouse
Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb.
Case DT, Burton SD, Gedeon JY, Williams SG, Urban NN, Seal RP
Nature communications (2017) 81: 652. 139 105 IHC; tested species: mouse
Nuclei-specific differences in nerve terminal distribution, morphology, and development in mouse visual thalamus.
Hammer S, Carrillo GL, Govindaiah G, Monavarfeshani A, Bircher JS, Su J, Guido W, Fox MA
Neural development (2014) 9: 16. 139 105 IHC
Neuregulin-1 is concentrated in the postsynaptic subsurface cistern of C-bouton inputs to α-motoneurons and altered during motoneuron diseases.
Gallart-Palau X, Tarabal O, Casanovas A, Sábado J, Correa FJ, Hereu M, Piedrafita L, Calderó J, Esquerda JE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014) 288: 3618-32. 139 105 IHC
Altered Dynein Axonemal Assembly Factor 1 Expression in C-Boutons in Bulbar and Spinal Cord Motor-Neurons in Sporadic Amyotrophic Lateral Sclerosis.
Andrés-Benito P, Povedano M, Torres P, Portero-Otín M, Ferrer I
Journal of neuropathology and experimental neurology (2019) : . 139 105 IHC-P; tested species: human
WB
ICC
IHC
IHC
IHC-P
Background
The vesicular acetylcholine transporter VAChT is an integral membrane protein with 12 putative trans-membrane domains. VAChT and choline acetyltransferase (ChAT) are encoded by genes organized in a single gene locus, and coregulation of the two genes has been reported several times.
VAChT translocates acetylcholine from the cytoplasm into synaptic vesicles where it stays until release. After release from the presynaptic nerve terminal acetylcholine is hydrolyzed by acetylcholine esterase. During Alzheimer´s disease acetylcholine is one of the first neurotransmitters to be reduced.
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