VAChT protein - 139-1P

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles

control protein

Cat. No.: 139-1P

Amount: 100 µg

Price: $105.00

Cat. No. 139-1P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	139 103, 139 105, 139 017, 139 308
Remarks	This control protein consists of the Strep-Tag® fusion protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	139-1p.pdf

Cat. No.: 139-1P

Amount: 100 µg

Price: $105.00

Background

The vesicular acetylcholine transporter VAChT is an integral membrane protein with 12 putative trans-membrane domains. VAChT and choline acetyltransferase (ChAT) are encoded by genes organized in a single gene locus, and coregulation of the two genes has been reported several times.
VAChT translocates acetylcholine from the cytoplasm into synaptic vesicles where it stays until release. After release from the presynaptic nerve terminal acetylcholine is hydrolyzed by acetylcholine esterase. During Alzheimer´s disease acetylcholine is one of the first neurotransmitters to be reduced.

1 ) Roghani A et al., Brain Res. Mol. Brain Res. (2002)
2 ) Dolezal V et al., Brain Res. Bull. (2001)
3 ) Mallet J et al., J. Physiol. Paris (1998)
4 ) Li JY et al., Neurochem. Int. ()
5 ) Arvidsson U et al., J. Comp. Neurol. (1997)

Protocols

OTHER

Preadsorbtion

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