Tailor-made Antibodies
and Tools for Life Science
- will be discontinued soon
Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received rabbit polyclonal VAChT antibody 139 103. It will be replaced by our new rabbit monoclonal recombinant IgG VAChT antibody 139 008.

VAChT antibody - 139 103 K.O.

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 139 103
Amount: 50 µg
Price: $410.00
Cat. No. 139 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 10000 gallery  
IHC: 1 : 100 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
iDISCO: 1 : 500
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: human (Q16572), rat (Q62666), mouse (O35304), pig, zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 24027290
Matching control protein/peptide 139-1P
Remarks

This antibody detects the glycosylated and unglycosylated protein and is an excellent marker for cholinergic axons.
WB:  VAChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 139_103.pdf

References for VAChT - 139 103

M1 muscarinic receptor activation reverses age-related memory updating impairment in mice.
Jardine KH, Minard EP, Wideman CE, Edwards H, Abouelnaga KH, Messer WS, Winters BD
Neurobiology of aging (2024) 145: 65-75. 139 103 WB; tested species: mouse
Cholinergic signaling via muscarinic M1 receptor confers resistance to docetaxel in prostate cancer.
Wang J, Wei J, Pu T, Zeng A, Karthikeyan V, Bechtold B, Vo K, Chen J, Lin TP, Chang AP, Corey E, et al.
Cell reports. Medicine (2024) : 101388. 139 103 WB, IHC; tested species: human,mouse
Enriched environment attenuates hippocampal theta and gamma rhythms dysfunction in chronic cerebral hypoperfusion via improving imbalanced neural afferent levels.
Zheng J, Peng S, Cui L, Liu X, Li T, Zhao Z, Li Y, Hu Y, Zhang M, Xu L, Zhang J, et al.
Frontiers in cellular neuroscience (2023) 17: 985246. 139 103 WB, IHC; tested species: rat
Continuous cholinergic-dopaminergic updating in the nucleus accumbens underlies approaches to reward-predicting cues.
Skirzewski M, Princz-Lebel O, German-Castelan L, Crooks AM, Kim GK, Tarnow SH, Reichelt A, Memar S, Palmer D, Li Y, Jane Rylett R, et al.
Nature communications (2022) 131: 7924. 139 103 WB, IHC; KO verified; tested species: mouse
Cholinergic transmission from the basal forebrain modulates social memory in male mice.
Kljakic O, Al-Onaizi M, Janíčková H, Chen KS, Guzman MS, Prado MAM, Prado VF
The European journal of neuroscience (2021) 546: 6075-6092. 139 103 WB; KO verified; tested species: mouse
Motoneuron-specific loss of VAChT mimics neuromuscular defects seen in congenital myasthenic syndrome.
Joviano-Santos JV, Kljakic O, Magalhães-Gomes MPS, Valadão PAC, de Oliveira LR, Prado MAM, Prado VF, Guatimosim C
The FEBS journal (2021) 28818: 5331-5349. 139 103 WB; KD verified; tested species: mouse
Vesicular Acetylcholine Transporter Alters Cholinergic Tone and Synaptic Plasticity in DYT1 Dystonia.
Tassone A, Martella G, Meringolo M, Vanni V, Sciamanna G, Ponterio G, Imbriani P, Bonsi P, Pisani A
Movement disorders : official journal of the Movement Disorder Society (2021) : . 139 103 WB, IHC; tested species: mouse
Behavioral, neuromorphological, and neurobiochemical effects induced by omega-3 fatty acids following basal forebrain cholinergic depletion in aged mice.
Cutuli D, Landolfo E, Nobili A, De Bartolo P, Sacchetti S, Chirico D, Marini F, Pieroni L, Ronci M, D'Amelio M, D'Amato FR, et al.
Alzheimer's research & therapy (2020) 121: 150. 139 103 WB; tested species: mouse
Increased cholinergic activity under conditions of low estrogen leads to adverse cardiac remodeling.
Teixeira VP, Miranda K, Scalzo S, Rocha-Resende C, Silva MM, Tezini GCSV, Melo MB, Souza-Neto FP, Silva KSC, Jesus ICG, Santos AK, et al.
American journal of physiology. Cell physiology (2020) : . 139 103 WB, ICC; tested species: mouse
Neuroprotective Role of Dietary Supplementation with Omega-3 Fatty Acids in the Presence of Basal Forebrain Cholinergic Neurons Degeneration in Aged Mice.
Cutuli D, Landolfo E, Decandia D, Nobili A, Viscomi MT, La Barbera L, Sacchetti S, De Bartolo P, Curci A, D'Amelio M, Farioli-Vecchioli S, et al.
International journal of molecular sciences (2020) 215: . 139 103 WB, IHC; tested species: mouse
Age-related neurochemical and behavioural changes in D409V/WT GBA1 mouse: Relevance to lewy body dementia.
Clarke E, Jantrachotechatchawan C, Buhidma Y, Broadstock M, Yu L, Howlett D, Aarsland D, Ballard C, Francis PT
Neurochemistry international (2019) 129: 104502. 139 103 WB, IHC; tested species: mouse
Altered motor, anxiety-related and attentional task performance at baseline associate with multiple gene copies of the vesicular acetylcholine transporter and related protein overexpression in ChAT::Cre+ rats.
Mantanona CP, Alsiö J, Elson JL, Fisher BM, Dalley JW, Bussey T, Pienaar IS
Brain structure & function (2019) : . 139 103 WB; tested species: rat
Selective decrease of cholinergic signaling from pedunculopontine and laterodorsal tegmental nuclei has little impact on cognition but markedly increases susceptibility to stress.
Janickova H, Kljakic O, Rosborough K, Raulic S, Matovic S, Gros R, Saksida LM, Bussey TJ, Inoue W, Prado VF, Prado MAM, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201802108R. 139 103 WB, IHC; KD verified; tested species: mouse
Cholinergic innervation of principal neurons in the cochlear nucleus of the Mongolian gerbil.
Gillet C, Goyer D, Kurth S, Griebel H, Kuenzel T
The Journal of comparative neurology (2018) 52610: 1647-1661. 139 103 WB, IHC
Deletion of the vesicular acetylcholine transporter from pedunculopontine/laterodorsal tegmental neurons modifies gait.
Janickova H, Rosborough K, Al-Onaizi M, Kljakic O, Guzman MS, Gros R, Prado MA, Prado VF
Journal of neurochemistry (2017) 1405: 787-798. 139 103 WB; tested species: mouse
Cardiac acetylcholine inhibits ventricular remodeling and dysfunction under pathologic conditions.
Roy A, Dakroub M, Tezini GC, Liu Y, Guatimosim S, Feng Q, Salgado HC, Prado VF, Prado MA, Gros R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2016) 302: 688-701. 139 103 WB, IHC
An essential role of acetylcholine-glutamate synergy at habenular synapses in nicotine dependence.
Frahm S, Antolin-Fontes B, Görlich A, Zander JF, Ahnert-Hilger G, Ibañez-Tallon I
eLife (2015) 4: e11396. 139 103 WB, IHC
MeCP2 in the enteric nervous system.
Wahba G, Schock SC, Claridge E, Bettolli M, Grynspan D, Humphreys P, Staines WA
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2015) 278: 1156-61. 139 103 WB
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 139 103 WB; tested species: mouse
Severe drug-induced repetitive behaviors and striatal overexpression of VAChT in ChAT-ChR2-EYFP BAC transgenic mice.
Crittenden JR, Lacey CJ, Lee T, Bowden HA, Graybiel AM
Frontiers in neural circuits (2014) 8: 57. 139 103 WB; tested species: mouse
Forebrain deletion of the vesicular acetylcholine transporter results in deficits in executive function, metabolic, and RNA splicing abnormalities in the prefrontal cortex.
Kolisnyk B, Al-Onaizi MA, Hirata PH, Guzman MS, Nikolova S, Barbash S, Soreq H, Bartha R, Prado MA, Prado VF
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3337: 14908-20. 139 103 WB; KO verified; tested species: mouse
ChAT-ChR2-EYFP mice have enhanced motor endurance but show deficits in attention and several additional cognitive domains.
Kolisnyk B, Guzman MS, Raulic S, Fan J, Magalhães AC, Feng G, Gros R, Prado VF, Prado MA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3325: 10427-38. 139 103 WB
Novel strains of mice deficient for the vesicular acetylcholine transporter: insights on transcriptional regulation and control of locomotor behavior.
Martins-Silva C, De Jaeger X, Guzman MS, Lima RD, Santos MS, Kushmerick C, Gomez MV, Caron MG, Prado MA, Prado VF
PloS one (2011) 63: e17611. 139 103 WB, IHC; tested species: mouse
The vesicular acetylcholine transporter is required for neuromuscular development and function.
de Castro BM, De Jaeger X, Martins-Silva C, Lima RD, Amaral E, Menezes C, Lima P, Neves CM, Pires RG, Gould TW, Welch I, et al.
Molecular and cellular biology (2009) 2919: 5238-50. 139 103 WB
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 139 103 WB; tested species: rat
The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone.
Gras C, Amilhon B, Lepicard EM, Poirel O, Vinatier J, Herbin M, Dumas S, Tzavara ET, Wade MR, Nomikos GG, Hanoun N, et al.
Nature neuroscience (2008) 113: 292-300. 139 103 WB
Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2005) 956: 1732-45. 139 103 WB; tested species: rat
Vesicular localization and activity-dependent trafficking of presynaptic choline transporters.
Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2003) 2330: 9697-709. 139 103 WB, IHC; tested species: mouse
Cat. No.: 139 103
Amount: 50 µg
Price: $410.00