Tailor-made Antibodies
and Tools for Life Science
- will be discontinued soon
Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received Guinea pig polyclonal VAChT antibody 139 105. It will be replaced by our new Guinea pig monoclonal recombinant IgG VAChT antibody 139 308.

VAChT antibody - 139 105

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles
Guinea pig polyclonal purified antibody
Cat. No.: 139 105
Amount: 50 µg
Price: $480.00
Cat. No. 139 105 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
iDISCO: 1 : 400
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: rat (Q62666), mouse (O35304), human (Q16572).
Other species not tested yet.
Matching control protein/peptide 139-1P
Remarks

This antibody is less sensitive compared to the rabbit antibody.
WB: VAChT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Data sheet 139_105.pdf

References for VAChT - 139 105

Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Tetzlaff SK, Reyhan E, Layer N, Bengtson CP, Heuer A, Schroers J, Faymonville AJ, Langeroudi AP, Drewa N, Keifert E, Wagner J, et al.
Cell (2024) : . 139 105 ICC, IHC-P; tested species: human,mouse
Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody.
Fabris F, Šoštarić P, Matak I, Binz T, Toffan A, Simonato M, Montecucco C, Pirazzini M, Rossetto O
International journal of molecular sciences (2022) 238: . 139 105 ICC; tested species: rat
Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.
Djemil S, Ressel CR, Abdel-Ghani M, Schneeweis AK, Pak DTS
Cellular and molecular neurobiology (2020) : . 139 105 ICC; tested species: rat
Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 139 105 ICC; tested species: mouse
Neuregulin 1-ErbB module in C-bouton synapses on somatic motor neurons: molecular compartmentation and response to peripheral nerve injury.
Casanovas A, Salvany S, Lahoz V, Tarabal O, Piedrafita L, Sabater R, Hernández S, Calderó J, Esquerda JE
Scientific reports (2017) 7: 40155. 139 105 ICC
Cat. No.: 139 105
Amount: 50 µg
Price: $480.00