USP9x antibody - 285 003

USP9x is a deubiquitinase belonging to the UBP family

Rabbit polyclonal purified antibody

Cat. No.: 285 003

Amount: 50 µg

Price: $455.00

Cat. No. 285 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 91 from mouse USP9x (UniProt Id: P70398)
Reactivity	Reacts with: rat, mouse (P70398). Other species not tested yet.
Data sheet	285_003.pdf

Cat. No.: 285 003

Amount: 50 µg

Price: $455.00

Background

Ubiquitinylation of proteins regulates their stability and turnover and is involved in many cellular processes like endocytosis, cell-cycle regulation, transcriptional activation, etc.
Ubiquitin can be removed by deubiquitylating enzymes (DUBs) comprising ubiquitin specific proteases (USPs), or ubiquitin C-terminal hydrolases (UCHs).
Fat facets in mouse (FAM, also known as Usp9X) is a developmentally regulated UBP modulating ubiquitinylation of proteins like β-catenin, α-synuclein and others.

1 ) Rott R et al., Proc. Natl. Acad. Sci. U.S.A. (2011)
2 ) Xie Y et al., J. Biol. Chem. (2013)
3 ) Mouchantaf R et al., J. Biol. Chem. (2006)
4 ) Murray RZ et al., Mol. Biol. Cell (2004)

Protocols

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