Synaptic Systems - Syntaxin5

Cat. No.: 110-5P

Amount: 100 µg

Price: $105.00

Background

Syntaxin 5, a member of the SNARE family of proteins, is functionally related to the yeast protein Sed5p. Two syntaxin 5 isoforms (35 and 42 kDa) have been described which are generated from the same mRNA by alternative translation initiation. The longer 42 kDa variant contains an N-terminal extension carrying a putative type II ER-retrieval signal.
Syntaxin 5 forms a SNARE complex with membrin, GOSR 1, rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GOSR 1 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1.
Recently syntaxin 5 has been shown to be involved in the processing and accumulation of β-APP in neuronal cells.

1 ) Suga K et al., J. Neurochem. (2005)
2 ) Tai G et al., Mol. Biol. Cell (2004)
3 ) Williams AL et al., Mol. Biol. Cell (2004)
4 ) Xu Y et al., Mol. Biol. Cell (2002)
5 ) Zhang T et al., J. Biol. Chem. (2001)
6 ) Rabouille C et al., Cell (1998)
7 ) Rowe T et al., Science (1998)
8 ) Hui N et al., Mol. Biol. Cell (1997)
9 ) Ravichandran V et al., J. Mol. Neurosci. (1997)
10 ) Dascher C et al., J. Biol. Chem. (1996)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 110-5P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 1 to 279 from mouse Syntaxin5 (UniProt Id: Q8K1E0)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	110 053, 110 053BT
Remarks	This control protein consists of the recombinant protein (aa 1-279) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	110-5p.pdf

Syntaxin5 protein - 110-5P