Synaptic Systems

Cat. No.: 170 003

Amount: 50 µg

Price: $375.00

Cat. No. 170 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 190 from human Membrin (UniProt Id: O14653)
Reactivity	Reacts with: rat (O35165), mouse (O35166), human (O14653). Other species not tested yet.
Data sheet	170_003.pdf

References for Membrin - 170 003

BET1 variants establish impaired vesicular transport as a cause for muscular dystrophy with epilepsy.
Donkervoort S, Krause N, Dergai M, Yun P, Koliwer J, Gorokhova S, Geist Hauserman J, Cummings BB, Hu Y, Smith R, Uapinyoying P, et al.
EMBO molecular medicine (2021) : e13787. 170 003 WB, ICC; tested species: human,mouse

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 170 003 WB; tested species: human

COG6 interacts with a subset of the Golgi SNAREs and is important for the Golgi complex integrity.
Kudlyk T, Willett R, Pokrovskaya ID, Lupashin V
Traffic (Copenhagen, Denmark) (2013) 142: 194-204. 170 003 WB

Cat. No.: 170 003

Amount: 50 µg

Price: $375.00

ICC

Background

Membrin also known as GOSR 2, GS 27 and GOS 27 is a 27 kDa membrane protein on the surface of the Golgi compartment. It has been shown to serve as a tSNARE in ER to Golgi transport.
In co-immunoprecipitation studies membrin was identified as a member of a SNARE complex consisting of syntaxin 5, GOSR 1 (GS 28, GOS 28), rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GS 28 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1. It has been suggested that theses complexes mediate the fusion of ER-derived vesicles with vesicular tubular clusters (VTC), and the fusion of VTCs to form the cis-Golgi compartment.

1 ) Honda A et al., J. Cell Biol. (2005)
2 ) Volchuk A et al., Mol. Biol. Cell (2004)
3 ) Joglekar AP et al., J. Biol. Chem. (2003)
4 ) Shorter J et al., J. Cell Biol. (2002)
5 ) Xu D et al., J. Biol. Chem. (2000)
6 ) Chao DS et al., J. Cell Biol. (1999)
7 ) Hay JC et al., J. Cell Biol. (1998)
8 ) Rowe T et al., Science (1998)

Protocols

Standard protocol

ICC

Membrin antibody - 170 003

References for Membrin - 170 003