|Cat. No. 170 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 100 up to 1 : 1000 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 2 to 190 from rat Membrin (UniProt Id: O35165)|
Reacts with: rat (O35165), mouse (O35166).
Other species not tested yet.
|Specificity||Specific for membrin.|
Membrin also known as GOSR 2, GS 27 and GOS 27 is a 27 kDa membrane protein on the surface of the Golgi compartment. It has been shown to serve as a tSNARE in ER to Golgi transport.
In co-immunoprecipitation studies membrin was identified as a member of a SNARE complex consisting of syntaxin 5, GOSR 1 (GS 28, GOS 28), rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GS 28 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1. It has been suggested that theses complexes mediate the fusion of ER-derived vesicles with vesicular tubular clusters (VTC), and the fusion of VTCs to form the cis-Golgi compartment.