Cat. No.: 110 161
Amount: 100 µg
Price:
$415.00
Cat. No. 110 161 |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not recommended Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not recommended Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.', $event)" style="cursor: help;">ELISA: yes (see remarks) |
Clone | 148.6 |
Subtype | IgG2b (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 302 from rat Syntaxin16 (UniProt Id: A0A0G2K528) |
Reactivity |
Reacts with: rat (A0A0G2K528). No signal: zebrafish. Other species not tested yet. |
Specificity | K.D. validated PubMed: 23626741 |
Matching control protein/peptide | 110-16P |
Remarks |
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 110 163 as detector antibody. The ELISA-protocol for membrane proteins is recommended. |
Data sheet | 110_161.pdf |
Syntaxin 16, a member of the SNARE family of proteins, localizes to the Golgi stack. It has been shown to be involved in trans-Golgi network trafficking and to interact with VAMP 3, VAMP 4 and VAMP 8.
Four splice variants (syntaxin 16a, b, c, d) have been described, which may have different roles in intracellular trafficking. The splice variant c is the shortest and localizes to the cytoplasm.