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Home > Products > 110 163

Syntaxin16 antibody - 110 163

Syntaxin16 is a SNARE protein involved in trans-Golgi network trafficking
Rabbit polyclonal purified antibody
Cat. No.: 110 163
Amount: 50 µg
Price: $455.00
Cat. No. 110 163 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen Recombinant protein corresponding to AA 1 to 302 from rat Syntaxin16 (UniProt Id: A0A0G2K528)
Reactivity Reacts with: human (O14662), rat (A0A0G2K528), mouse (Q8BVI5), hamster.
Other species not tested yet.
Specificity Recognizes all four isoforms syntaxin 16a, b, c, d.
Matching control protein/peptide 110-16P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 161 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 110_163.pdf

References for Syntaxin16 - 110 163

Sugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts.
Ng PY, Ribet ABP, Guo Q, Mullin BH, Tan JWY, Landao-Bassonga E, Stephens S, Chen K, Yuan J, Abudulai L, Bollen M, et al.
Nature communications (2023) 141: 906. 110 163 WB; tested species: mouse
Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 110 163 ICC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 110 163 DNA-PAINT; tested species: rat
Cat. No.: 110 163
Amount: 50 µg
Price: $455.00
Sugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts.
Ng PY, Ribet ABP, Guo Q, Mullin BH, Tan JWY, Landao-Bassonga E, Stephens S, Chen K, Yuan J, Abudulai L, Bollen M, et al.
Nature communications (2023) 141: 906. 110 163 WB; tested species: mouse
Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 110 163 ICC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 110 163 DNA-PAINT; tested species: rat
WB
ICC
Background

Syntaxin 16, a member of the SNARE family of proteins, localizes to the Golgi stack. It has been shown to be involved in trans-Golgi network trafficking and to interact with VAMP 3, VAMP 4 and VAMP 8.
Four splice variants (syntaxin 16a, b, c, d) have been described, which may have different roles in intracellular trafficking. The splice variant c is the shortest and localizes to the cytoplasm.
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