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Syntaxin1A antibody - 110 302 K.O.

Syntaxin1 is a plasma membrane SNARE protein essential for synaptic vesicle fusion
Rabbit polyclonal antiserum
Cat. No.: 110 302
Amount: 200 µl
Price: $355.00
Cat. No. 110 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: not tested yet
IHC-P: not tested yet
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 172 to 189 from rat Syntaxin1A (UniProt Id: P32851)
Reactivity Reacts with: human (Q16623), rat (P32851), mouse (O35526), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for syntaxin 1A, no cross reactivity to syntaxin 1B. K.O. validated PubMed: 28031464
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.
Data sheet 110_302.pdf

References for Syntaxin1A - 110 302

Analysis of the neuromuscular deficits caused by STAM1 deficiency.
McLean JW, VanHart M, McWilliams MP, Farmer CB, Crossman DK, Cowell RM, Wilson JA, Wilson SM
Current research in neurobiology (2024) 7: 100138. 110 302 WB; tested species: mouse
Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B.
Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F
Cell death discovery (2023) 91: 187. 110 302 WB, IP; tested species: mouse
A novel synaptopathy-defective synaptic vesicle protein trafficking in the mutant CHMP2B mouse model of frontotemporal dementia.
Clayton EL, Bonnycastle K, Isaacs AM, Cousin MA, Schorge S
Journal of neurochemistry (2022) 1603: 412-425. 110 302 WB; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 110 302 IP, WB; tested species: human,mouse
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning.
Santa-Marinha L, Castanho I, Silva RR, Bravo FV, Miranda AM, Meira T, Morais-Ribeiro R, Marques F, Xu Y, Point du Jour K, Wenk M, et al.
Cell reports (2020) 3012: 4197-4208.e6. 110 302 WB; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 110 302 WB; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 110 302 WB; tested species: mouse
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 110 302 WB; tested species: rat
New Roles of Syntaxin-1A in Insulin Granule Exocytosis and Replenishment.
Liang T, Qin T, Xie L, Dolai S, Zhu D, Prentice KJ, Wheeler M, Kang Y, Osborne L, Gaisano HY
The Journal of biological chemistry (2017) 2926: 2203-2216. 110 302 WB, IHC; KO verified; tested species: mouse
PRRT2 Is a Key Component of the Ca(2+)-Dependent Neurotransmitter Release Machinery.
Valente P, Castroflorio E, Rossi P, Fadda M, Sterlini B, Cervigni RI, Prestigio C, Giovedì S, Onofri F, Mura E, Guarnieri FC, et al.
Cell reports (2016) 151: 117-31. 110 302 WB
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 302 WB, IP; tested species: mouse
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 110 302 WB, ICC; tested species: human
Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells.
Sander LE, Frank SP, Bolat S, Blank U, Galli T, Bigalke H, Bischoff SC, Lorentz A
European journal of immunology (2008) 383: 855-63. 110 302 WB; tested species: human,rat
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles.
Martinez-Arca S, Proux-Gillardeaux V, Alberts P, Louvard D, Galli T
Journal of cell science (2003) 116Pt 13: 2805-16. 110 302 WB, ICC
Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B.
Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F
Cell death discovery (2023) 91: 187. 110 302 WB, IP; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 110 302 IP, WB; tested species: human,mouse
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 302 WB, IP; tested species: mouse
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 110 302 ICC; tested species: mouse
Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.
Chen Y, Wang YH, Zheng Y, Li M, Wang B, Wang QW, Fu CL, Liu YN, Li X, Yao J
Cell reports (2021) 3411: 108842. 110 302 ICC; tested species: mouse
Microglial expression of GAT-1 in the cerebral cortex.
Fattorini G, Catalano M, Melone M, Serpe C, Bassi S, Limatola C, Conti F
Glia (2020) 683: 646-655. 110 302 ICC; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 110 302 ICC; tested species: rat
Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains.
Milovanovic D, Honigmann A, Koike S, Göttfert F, Pähler G, Junius M, Müllar S, Diederichsen U, Janshoff A, Grubmüller H, Risselada HJ, et al.
Nature communications (2015) 6: 5984. 110 302 ICC
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 110 302 ICC; tested species: rat
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 110 302 WB, ICC; tested species: human
Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.
Andersson SA, Olsson AH, Esguerra JL, Heimann E, Ladenvall C, Edlund A, Salehi A, Taneera J, Degerman E, Groop L, Ling C, et al.
Molecular and cellular endocrinology (2012) 3641-2: 36-45. 110 302 ICC
The SNAP-25 linker as an adaptation toward fast exocytosis.
Nagy G, Milosevic I, Mohrmann R, Wiederhold K, Walter AM, Sørensen JB
Molecular biology of the cell (2008) 199: 3769-81. 110 302 ICC
Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis.
De Blas GA, Roggero CM, Tomes CN, Mayorga LS
PLoS biology (2005) 310: e323. 110 302 ICC
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles.
Martinez-Arca S, Proux-Gillardeaux V, Alberts P, Louvard D, Galli T
Journal of cell science (2003) 116Pt 13: 2805-16. 110 302 WB, ICC
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs () 112: 305-321. 110 302 ICC; tested species: mouse
New Roles of Syntaxin-1A in Insulin Granule Exocytosis and Replenishment.
Liang T, Qin T, Xie L, Dolai S, Zhu D, Prentice KJ, Wheeler M, Kang Y, Osborne L, Gaisano HY
The Journal of biological chemistry (2017) 2926: 2203-2216. 110 302 WB, IHC; KO verified; tested species: mouse
Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors.
Cartier E, Hamilton PJ, Belovich AN, Shekar A, Campbell NG, Saunders C, Andreassen TF, Gether U, Veenstra-Vanderweele J, Sutcliffe JS, Ulery-Reynolds PG, et al.
EBioMedicine (2015) 22: 135-146. 110 302 IHC; tested species: mouse
Cat. No.: 110 302
Amount: 200 µl
Price: $355.00
Analysis of the neuromuscular deficits caused by STAM1 deficiency.
McLean JW, VanHart M, McWilliams MP, Farmer CB, Crossman DK, Cowell RM, Wilson JA, Wilson SM
Current research in neurobiology (2024) 7: 100138. 110 302 WB; tested species: mouse
Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B.
Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F
Cell death discovery (2023) 91: 187. 110 302 WB, IP; tested species: mouse
A novel synaptopathy-defective synaptic vesicle protein trafficking in the mutant CHMP2B mouse model of frontotemporal dementia.
Clayton EL, Bonnycastle K, Isaacs AM, Cousin MA, Schorge S
Journal of neurochemistry (2022) 1603: 412-425. 110 302 WB; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 110 302 IP, WB; tested species: human,mouse
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning.
Santa-Marinha L, Castanho I, Silva RR, Bravo FV, Miranda AM, Meira T, Morais-Ribeiro R, Marques F, Xu Y, Point du Jour K, Wenk M, et al.
Cell reports (2020) 3012: 4197-4208.e6. 110 302 WB; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 110 302 WB; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 110 302 WB; tested species: mouse
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 110 302 WB; tested species: rat
New Roles of Syntaxin-1A in Insulin Granule Exocytosis and Replenishment.
Liang T, Qin T, Xie L, Dolai S, Zhu D, Prentice KJ, Wheeler M, Kang Y, Osborne L, Gaisano HY
The Journal of biological chemistry (2017) 2926: 2203-2216. 110 302 WB, IHC; KO verified; tested species: mouse
PRRT2 Is a Key Component of the Ca(2+)-Dependent Neurotransmitter Release Machinery.
Valente P, Castroflorio E, Rossi P, Fadda M, Sterlini B, Cervigni RI, Prestigio C, Giovedì S, Onofri F, Mura E, Guarnieri FC, et al.
Cell reports (2016) 151: 117-31. 110 302 WB
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 302 WB, IP; tested species: mouse
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 110 302 WB, ICC; tested species: human
Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells.
Sander LE, Frank SP, Bolat S, Blank U, Galli T, Bigalke H, Bischoff SC, Lorentz A
European journal of immunology (2008) 383: 855-63. 110 302 WB; tested species: human,rat
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles.
Martinez-Arca S, Proux-Gillardeaux V, Alberts P, Louvard D, Galli T
Journal of cell science (2003) 116Pt 13: 2805-16. 110 302 WB, ICC
Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B.
Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F
Cell death discovery (2023) 91: 187. 110 302 WB, IP; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 110 302 IP, WB; tested species: human,mouse
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 302 WB, IP; tested species: mouse
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 110 302 ICC; tested species: mouse
Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.
Chen Y, Wang YH, Zheng Y, Li M, Wang B, Wang QW, Fu CL, Liu YN, Li X, Yao J
Cell reports (2021) 3411: 108842. 110 302 ICC; tested species: mouse
Microglial expression of GAT-1 in the cerebral cortex.
Fattorini G, Catalano M, Melone M, Serpe C, Bassi S, Limatola C, Conti F
Glia (2020) 683: 646-655. 110 302 ICC; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 110 302 ICC; tested species: rat
Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains.
Milovanovic D, Honigmann A, Koike S, Göttfert F, Pähler G, Junius M, Müllar S, Diederichsen U, Janshoff A, Grubmüller H, Risselada HJ, et al.
Nature communications (2015) 6: 5984. 110 302 ICC
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 110 302 ICC; tested species: rat
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 110 302 WB, ICC; tested species: human
Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.
Andersson SA, Olsson AH, Esguerra JL, Heimann E, Ladenvall C, Edlund A, Salehi A, Taneera J, Degerman E, Groop L, Ling C, et al.
Molecular and cellular endocrinology (2012) 3641-2: 36-45. 110 302 ICC
The SNAP-25 linker as an adaptation toward fast exocytosis.
Nagy G, Milosevic I, Mohrmann R, Wiederhold K, Walter AM, Sørensen JB
Molecular biology of the cell (2008) 199: 3769-81. 110 302 ICC
Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis.
De Blas GA, Roggero CM, Tomes CN, Mayorga LS
PLoS biology (2005) 310: e323. 110 302 ICC
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles.
Martinez-Arca S, Proux-Gillardeaux V, Alberts P, Louvard D, Galli T
Journal of cell science (2003) 116Pt 13: 2805-16. 110 302 WB, ICC
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs () 112: 305-321. 110 302 ICC; tested species: mouse
New Roles of Syntaxin-1A in Insulin Granule Exocytosis and Replenishment.
Liang T, Qin T, Xie L, Dolai S, Zhu D, Prentice KJ, Wheeler M, Kang Y, Osborne L, Gaisano HY
The Journal of biological chemistry (2017) 2926: 2203-2216. 110 302 WB, IHC; KO verified; tested species: mouse
Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors.
Cartier E, Hamilton PJ, Belovich AN, Shekar A, Campbell NG, Saunders C, Andreassen TF, Gether U, Veenstra-Vanderweele J, Sutcliffe JS, Ulery-Reynolds PG, et al.
EBioMedicine (2015) 22: 135-146. 110 302 IHC; tested species: mouse
WB
ICC
Background

Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca2+-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.
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