Synaptic Systems - Synapsin1

Cat. No.: 106 011C3

Amount: 100 µg

Price: $475.00

Neuronal differentiation requires BRAT1 complex to remove REST from chromatin.
Dokaneheifard S, Gomes Dos Santos H, Guiselle Valencia M, Arigela H, Edupuganti RR, Shiekhattar R
Proceedings of the National Academy of Sciences of the United States of America (2024) 12123: e2318740121. 106 011C3 ICC; tested species: human

Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks.
Lantoine J, Procès A, Villers A, Halliez S, Buée L, Ris L, Gabriele S
ACS chemical neuroscience (2021) 1220: 3885-3897. 106 011C3 ICC; tested species: rat

ICC

IHC

Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.

1 ) Hosaka M et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Rosahl TW et al., Nature (1995)
4 ) Südhof TC et al., Nature (1995)

Protocols

ICC

IHC

Other products for this target

Cat. No. 106 011C3	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 100 up to 1 : 2000 gallery IHC: 1 : 100 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Sulfo-Cyanine 3
Clone	46.1
Subtype	IgG1
Immunogen	full-length recombinant rat Synapsin1 (UniProt Id: P09951)
Epitop	AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity	Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals. Weaker signal: zebrafish, chicken, other vertebrates. Other species not tested yet.
Specificity	Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated
Data sheet	106_011c3.pdf

Synapsin1 antibody - 106 011C3 K.O.

References for Synapsin1 - 106 011C3