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Home > Products > 119 111

SV2 B antibody - 119 111

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Mouse monoclonal purified IgG
Cat. No.: 119 111
Amount: 100 µg
Price: $415.00
Cat. No. 119 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 2000 up to 1 : 5000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
DNA-PAINT: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Clone 246E8
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat SV2B (UniProt Id: Q63564)
Reactivity Reacts with: human (Q7L1I2), rat (Q63564), mouse (Q8BG39).
Other species not tested yet.
Matching control protein/peptide 119-1P
Remarks

WB:  SV2 B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet 119_111.pdf

References for SV2 B - 119 111

Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 119 111 WB; tested species: mouse
Botulinum Neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations.
Pellett S, Tepp WH, Johnson EA
FEBS letters (2019) : . 119 111 WB; tested species: human
Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 119 111 WB
Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells.
Dagar S, Nagar S, Goel M, Cherukuri P, Dhingra NK
PloS one (2014) 93: e90250. 119 111 IHC, WB; tested species: mouse
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 119 111 IHC; tested species: mouse
Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells.
Dagar S, Nagar S, Goel M, Cherukuri P, Dhingra NK
PloS one (2014) 93: e90250. 119 111 IHC, WB; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 119 111 DNA-PAINT; tested species: rat
Cat. No.: 119 111
Amount: 100 µg
Price: $415.00
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 119 111 WB; tested species: mouse
Botulinum Neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations.
Pellett S, Tepp WH, Johnson EA
FEBS letters (2019) : . 119 111 WB; tested species: human
Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 119 111 WB
Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells.
Dagar S, Nagar S, Goel M, Cherukuri P, Dhingra NK
PloS one (2014) 93: e90250. 119 111 IHC, WB; tested species: mouse
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 119 111 IHC; tested species: mouse
Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells.
Dagar S, Nagar S, Goel M, Cherukuri P, Dhingra NK
PloS one (2014) 93: e90250. 119 111 IHC, WB; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 119 111 DNA-PAINT; tested species: rat
WB
ICC
IHC
IHC-P
Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.
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