Synaptic Systems

Cat. No.: 119 102

Amount: 200 µl

Price: $355.00

Cat. No. 119 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 200 up to 1 : 2000 gallery IHC: 1 : 200 up to 1 : 2000 gallery IHC-P: 1 : 200 gallery EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Synthetic peptide corresponding to AA 2 to 17 from rat SV2B (UniProt Id: Q63564)
Reactivity	Reacts with: human (Q7L1I2), rat (Q63564), mouse (Q8BG39), hamster, chicken. Other species not tested yet.
Specificity	K.O. validated PubMed: 19381277
Matching control protein/peptide	119-1P
Remarks	WB: SV2 B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	119_102.pdf

References for SV2 B - 119 102

SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 102 WB, IHC, EM; KO verified; tested species: mouse

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 119 102 WB; tested species: rat

Cohen Syndrome Patient iPSC-Derived Neurospheres and Forebrain-Like Glutamatergic Neurons Reveal Reduced Proliferation of Neural Progenitor Cells and Altered Expression of Synapse Genes.
Lee YK, Hwang SK, Lee SK, Yang JE, Kwak JH, Seo H, Ahn H, Lee YS, Kim J, Lim CS, Kaang BK, et al.
Journal of clinical medicine (2020) 96: . 119 102 WB, ICC; tested species: human

CNS synapses are stabilized trans-synaptically by laminins and laminin-interacting proteins.
Hunter DD, Manglapus MK, Bachay G, Claudepierre T, Dolan MW, Gesuelli KA, Brunken WJ
The Journal of comparative neurology (2019) 5271: 67-86. 119 102 WB, IHC; tested species: mouse

SV2B is essential for the integrity of the glomerular filtration barrier.
Fukusumi Y, Wakamatsu A, Takashima N, Hasegawa E, Miyauchi N, Tomita M, Kawachi H
Laboratory investigation; a journal of technical methods and pathology (2015) 955: 534-45. 119 102 WB, IHC, IP; KO verified; tested species: mouse

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 119 102 WB; tested species: mouse

Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E.
Mahrhold S, Strotmeier J, Garcia-Rodriguez C, Lou J, Marks JD, Rummel A, Binz T
The Biochemical journal (2013) 4531: 37-47. 119 102 WB

Tetanus toxin and botulinum toxin a utilize unique mechanisms to enter neurons of the central nervous system.
Blum FC, Chen C, Kroken AR, Barbieri JT
Infection and immunity (2012) 805: 1662-9. 119 102 WB

Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine.
Couesnon A, Molgó J, Connan C, Popoff MR
PLoS pathogens (2012) 83: e1002583. 119 102 WB

Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 119 102 WB, ICC

Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam.
Boido D, Farisello P, Cesca F, Ferrea E, Valtorta F, Benfenati F, Baldelli P
Neuroscience (2010) 1711: 268-83. 119 102 WB

Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina.
Morgans CW, Kensel-Hammes P, Hurley JB, Burton K, Idzerda R, McKnight GS, Bajjalieh SM
PloS one (2009) 44: e5230. 119 102 WB, IHC; KO verified; tested species: mouse

Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.
Rummel A, Häfner K, Mahrhold S, Darashchonak N, Holt M, Jahn R, Beermann S, Karnath T, Bigalke H, Binz T
Journal of neurochemistry (2009) 1106: 1942-54. 119 102 WB; tested species: mouse

Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers.
Couesnon A, Pereira Y, Popoff MR
Cellular microbiology (2008) 102: 375-87. 119 102 WB

Synaptic vesicle protein 2B is expressed in podocyte, and its expression is altered in proteinuric glomeruli.
Miyauchi N, Saito A, Karasawa T, Harita Y, Suzuki K, Koike H, Han GD, Shimizu F, Kawachi H
Journal of the American Society of Nephrology : JASN (2006) 1710: 2748-59. 119 102 WB, ICC, IHC

SV2A and SV2C contain a unique synaptotagmin-binding site.
Schivell AE, Mochida S, Kensel-Hammes P, Custer KL, Bajjalieh SM
Molecular and cellular neurosciences (2005) 291: 56-64. 119 102 WB

Brain synaptic junctional proteins at the acrosome of rat testicular germ cells.
Redecker P, Kreutz MR, Bockmann J, Gundelfinger ED, Boeckers TM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2003) 516: 809-19. 119 102 WB, IHC

SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 102 WB, IHC

Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice.
Hoffmann-Conaway S, Brockmann MM, Schneider K, Annamneedi A, Rahman KA, Bruns C, Textoris-Taube K, Trimbuch T, Smalla KH, Rosenmund C, Gundelfinger ED, et al.
eLife (2020) 9: . 119 102 ICC; tested species: mouse

Distinct synaptic vesicle recycling in inhibitory nerve terminals is coordinated by SV2A.
Bae JR, Lee W, Jo YO, Han S, Koh S, Song WK, Kim SH
Progress in neurobiology (2020) : 101879. 119 102 ICC; tested species: rat

Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS.
Aparicio GI, Formoso K, León A, Frasch AC, Scorticati C
Frontiers in synaptic neuroscience (2020) 12: 28. 119 102 ICC; tested species: rat

Physiological characterisation of human iPS-derived dopaminergic neurons.
Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS, Cowley SA, Wade-Martins R
PloS one (2014) 92: e87388. 119 102 ICC; tested species: human

Traffic of botulinum toxins A and E in excitatory and inhibitory neurons.
Verderio C, Grumelli C, Raiteri L, Coco S, Paluzzi S, Caccin P, Rossetto O, Bonanno G, Montecucco C, Matteoli M
Traffic (Copenhagen, Denmark) (2007) 82: 142-53. 119 102 ICC; tested species: rat

Mouse all-cone retina models of Cav1.4 synaptopathy.
Laird JG, Kopel A, Lankford CK, Baker SA
Frontiers in molecular neuroscience (2023) 16: 1155955. 119 102 IHC; tested species: mouse

Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 119 102 IHC; tested species: mouse

SMN controls neuromuscular junction integrity through U7 snRNP.
Tisdale S, Van Alstyne M, Simon CM, Mentis GZ, Pellizzoni L
Cell reports (2022) 4012: 111393. 119 102 IHC; tested species: mouse

Calcium is Reduced in Presynaptic Mitochondria of Motor Nerve Terminals during Neurotransmission in SMA Mice.
Lopez-Manzaneda M, Franco-Espin J, Tejero R, Cano R, Tabares L
Human molecular genetics (2021) : . 119 102 IHC; tested species: mouse

LIM-Homeodomain Transcription Factor LHX4 Is Required for the Differentiation of Retinal Rod Bipolar Cells and OFF-Cone Bipolar Subtypes.
Dong X, Yang H, Zhou X, Xie X, Yu D, Guo L, Xu M, Zhang W, Liang G, Gan L
Cell reports (2020) 3211: 108144. 119 102 IHC; tested species: mouse

Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis.
Crèvecoeur J, Kaminski RM, Rogister B, Foerch P, Vandenplas C, Neveux M, Mazzuferi M, Kroonen J, Poulet C, Martin D, Sadzot B, et al.
Neuropathology and applied neurobiology (2014) 402: 191-204. 119 102 IHC; tested species: human

Expression of SV2 isoforms during rodent brain development.
Crèvecœur J, Foerch P, Doupagne M, Thielen C, Vandenplas C, Moonen G, Deprez M, Rogister B
BMC neuroscience (2013) 14: 87. 119 102 IHC; KO verified; tested species: mouse

SV2 mediates entry of tetanus neurotoxin into central neurons.
Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER
PLoS pathogens (2010) 611: e1001207. 119 102 IHC; tested species: mouse

The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 119 102 IHC

SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 102 WB, IHC

Cat. No.: 119 102

Amount: 200 µl

Price: $355.00

Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers.
Couesnon A, Pereira Y, Popoff MR
Cellular microbiology (2008) 102: 375-87. 119 102 WB

SV2A and SV2C contain a unique synaptotagmin-binding site.
Schivell AE, Mochida S, Kensel-Hammes P, Custer KL, Bajjalieh SM
Molecular and cellular neurosciences (2005) 291: 56-64. 119 102 WB

SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 102 WB, IHC

Mouse all-cone retina models of Cav1.4 synaptopathy.
Laird JG, Kopel A, Lankford CK, Baker SA
Frontiers in molecular neuroscience (2023) 16: 1155955. 119 102 IHC; tested species: mouse

SMN controls neuromuscular junction integrity through U7 snRNP.
Tisdale S, Van Alstyne M, Simon CM, Mentis GZ, Pellizzoni L
Cell reports (2022) 4012: 111393. 119 102 IHC; tested species: mouse

SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 102 WB, IHC

ICC

IHC

IHC-P

Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.

1 ) Xu T et al., Nat. Cell Biol. (2001)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
4 ) Südhof TC et al., Nature (1995)
5 ) Bajjalieh SM et al., J. Neurosci. (1994)
6 ) Bajjalieh SM et al., Science (1992)
7 ) Feany MB et al., Cell (1992)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

SV2 B antibody - 119 102 K.O.

References for SV2 B - 119 102