Cat. No.: 111 111BT
Amount: 50 µg
Price:
$470.00
|
|
|
| Cat. No. 111 111BT |
50 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: yes (see remarks) ICC: 1 : 100 up to 1 : 500 IHC: 1 : 200 IHC-P: not tested yet ELISA: yes |
| Label | biotin |
| Clone | 71.2 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Full-length recombinant rat SNAP25B protein (UniProt Id: P60881-1) |
| Epitop |
AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1) |
| Reactivity |
Reacts with: human (P60880), rat (P60881), mouse (P60879), mammals. No signal: zebrafish. Other species not tested yet. |
| Specificity | Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. |
| Remarks |
IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein. |
| Data sheet | 111_111bt.pdf |
|
|
SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.