Cat. No.: 111-0P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 111-0P |
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized! |
| Applications |
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| Immunogen | Synthetic peptide corresponding to AA 192 to 206 from human SNAP25 (UniProt Id: P60880) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 111 002, 111 004 |
| Remarks |
This control peptide consists of the synthetic peptide (IDEANQRATKMLGSG) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 111-0p.pdf |
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SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.