SNAP25 antibody - 111 004

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis

Guinea pig polyclonal antiserum

Cat. No.: 111 004

Amount: 100 µl

Price: $365.00

Cat. No. 111 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 192 to 206 from human SNAP25 (UniProt Id: P60880)
Reactivity	Reacts with: human (P60880), rat (P60881), mouse (P60879), hamster, chicken, goldfish, zebrafish. Other species not tested yet.
Matching control protein/peptide	111-0P
Data sheet	111_004.pdf

Cat. No.: 111 004

Amount: 100 µl

Price: $365.00

Background

SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Fasshauer D et al., J. Biol. Chem. (1997)
5 ) Südhof TC et al., Nature (1995)
6 ) Blasi J et al., Nature (1993)
7 ) Prescott GR et al., BMC Neurosci (2011)
8 ) Schulze KL et al., Cell (1995)

Protocols

Standard protocol

OTHER

Preabsorbtion

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