Synaptic Systems - Synaptotagmin4

Cat. No.: 105-4P

Amount: 100 µg

Price: $105.00

Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin4 is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
The localization of synaptotagmin4 is still under discussion. A localization to synaptic vesicles (SVs) has been postulated but more recent studies suggest that it is present in the Golgi compartment, in distal parts of neurites and on large dense core vesicles (LDCVs) of NGF differentiated PC12 cells.

1 ) Ferguson GD et al., Hippocampus (2004)
2 ) Dai H et al., Nat. Struct. Mol. Biol. (2004)
3 ) Ferguson GD et al., Neuropharmacology (2004)
4 ) Zhang Q et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
5 ) Fukuda M et al., J. Biol. Chem. (2003)
6 ) Ibata K et al., Neurosci. Res. (2002)
7 ) Ferguson GD et al., Mol. Neurobiol. ()
8 ) Fukuda M et al., J. Neurochem. (2001)
9 ) Ibata K et al., J. Neurochem. (2000)
10 ) Thomas DM et al., Mol. Biol. Cell (1999)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 105-4P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 40 to 151 from rat Synaptotagmin4 (UniProt Id: P50232)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	105 043, 105 143
Remarks	This control protein consists of the recombinant protein (aa 40-151 of rat synaptotagmin 4) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	105-4p.pdf

Synaptotagmin4 protein - 105-4P