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Home > Products > 105 143

Synaptotagmin4 antibody - 105 143 K.O.

Synaptotagmin4 is located at the Golgi compartment and LDCVs in NGF differentiated PC12 cells
Rabbit polyclonal purified antibody
Cat. No.: 105 143
Amount: 50 µg
Price: $375.00
Cat. No. 105 143 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 40 to 151 from rat Synaptotagmin4 (UniProt Id: P50232)
Reactivity Reacts with: human (Q9H2B2), rat (P50232), mouse (P40749), hamster.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 105-4P
Remarks

WB: Cat. no. 105 043 is recommended for this application.
Data sheet 105_143.pdf

References for Synaptotagmin4 - 105 143

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 143 ICC, WB
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 143 ICC, WB
Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 143 ICC
Cat. No.: 105 143
Amount: 50 µg
Price: $375.00
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 143 ICC, WB
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 143 ICC, WB
Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 143 ICC
WB
ICC
IHC
Background
Up to now at least 17 synaptotagmins have been identified. Synaptotagmin 4 is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
The localization of synaptotagmin 4 is still under discussion. A localization to synaptic vesicles (SVs) has been postulated but more recent studies suggest that it is present in the Golgi compartment, in distal parts of neurites and on large dense core vesicles (LDCVs) of NGF differentiated PC12 cells.
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