Synaptic Systems - Synaptotagmin4

Cat. No.: 105 143

Amount: 50 µg

Price: $375.00

Cat. No. 105 143	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 40 to 151 from rat Synaptotagmin4 (UniProt Id: P50232)
Reactivity	Reacts with: human (Q9H2B2), rat (P50232), mouse (P40749), hamster. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	105-4P
Remarks	WB: Cat. no. 105 043 is recommended for this application.
Data sheet	105_143.pdf

References for Synaptotagmin4 - 105 143

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 143 ICC, WB

Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 143 ICC

Conserved expression of the zebrafish syt4 gene in GABAergic neurons in the cerebellum of adult fishes revealed by mammalian SYT4 immunoreactive-like signals.
Shiao MS, Liu ST, Siriwatcharapibool G, Thongpradit S, Khunpanich P, Tong SK, Huang CH, Jinawath N, Chou MY
Heliyon (2024) 109: e30575. 105 143 IHC; tested species: zebrafish

Cat. No.: 105 143

Amount: 50 µg

Price: $375.00

Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 143 ICC

ICC

IHC

Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin4 is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
The localization of synaptotagmin4 is still under discussion. A localization to synaptic vesicles (SVs) has been postulated but more recent studies suggest that it is present in the Golgi compartment, in distal parts of neurites and on large dense core vesicles (LDCVs) of NGF differentiated PC12 cells.

1 ) Ferguson GD et al., Hippocampus (2004)
2 ) Dai H et al., Nat. Struct. Mol. Biol. (2004)
3 ) Ferguson GD et al., Neuropharmacology (2004)
4 ) Zhang Q et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
5 ) Fukuda M et al., J. Biol. Chem. (2003)
6 ) Ibata K et al., Neurosci. Res. (2002)
7 ) Ferguson GD et al., Mol. Neurobiol. ()
8 ) Fukuda M et al., J. Neurochem. (2001)
9 ) Ibata K et al., J. Neurochem. (2000)
10 ) Thomas DM et al., Mol. Biol. Cell (1999)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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Synaptotagmin4 antibody - 105 143 K.O.

References for Synaptotagmin4 - 105 143