Synaptic Systems - Synaptotagmin4

Cat. No.: 105 043

Amount: 50 µg

Price: $375.00

Cat. No. 105 043	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: yes ICC: not recommended (see remarks) IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 40 to 151 from rat Synaptotagmin4 (UniProt Id: P50232)
Reactivity	Reacts with: rat (P50232), mouse (P40749). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	105-4P
Remarks	ICC: Cat. no. 105 143 is recommended.
Data sheet	105_043.pdf

References for Synaptotagmin4 - 105 043

REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion.
Prestigio C, Ferrante D, Marte A, Romei A, Lignani G, Onofri F, Valente P, Benfenati F, Baldelli P
eLife (2021) 10: . 105 043 WB; tested species: mouse

Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca2+ Sensitivity of Insulin Secretion Vesicles.
Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, et al.
Developmental cell (2018) 453: 347-361.e5. 105 043 WB, IHC; tested species: mouse

Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 043 WB; tested species: rat

Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis.
Tsai PS, Brewis IA, van Maaren J, Gadella BM
PloS one (2012) 73: e32603. 105 043 WB, ICC; tested species: pig

Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.
Andersson SA, Olsson AH, Esguerra JL, Heimann E, Ladenvall C, Edlund A, Salehi A, Taneera J, Degerman E, Groop L, Ling C, et al.
Molecular and cellular endocrinology (2012) 3641-2: 36-45. 105 043 WB

Cell therapy modulates expression of Tax1-binding protein 1 and synaptotagmin IV in a model of optic nerve lesion.
Mesentier-Louro LA, Coronel J, Zaverucha-do-Valle C, Mencalha A, Paredes BD, Abdelhay E, Mendez-Otero R, Santiago MF
Investigative ophthalmology & visual science (2012) 538: 4720-9. 105 043 WB, IHC

Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity.
Dean C, Liu H, Staudt T, Stahlberg MA, Vingill S, Bückers J, Kamin D, Engelhardt J, Jackson MB, Hell SW, Chapman ER, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5398-413. 105 043 WB, ICC

REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
Prada I, Marchaland J, Podini P, Magrassi L, D'Alessandro R, Bezzi P, Meldolesi J
The Journal of cell biology (2011) 1933: 537-49. 105 043 WB

Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons.
Arthur CP, Dean C, Pagratis M, Chapman ER, Stowell MH
Neuroscience (2010) 1671: 135-42. 105 043 WB, IP

Deferoxamine-induced neurite outgrowth and synapse formation in postnatal rat dorsal root ganglion (DRG) cell cultures.
Nowicki M, Kosacka J, Spanel-Borowski K, Borlak J
European journal of cell biology (2009) 8810: 551-62. 105 043 WB

Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release.
Dean C, Liu H, Dunning FM, Chang PY, Jackson MB, Chapman ER
Nature neuroscience (2009) 126: 767-76. 105 043 WB, app.icc; ip

Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM

Neuropeptide exocytosis involving synaptotagmin-4 and oxytocin in hypothalamic programming of body weight and energy balance.
Zhang G, Bai H, Zhang H, Dean C, Wu Q, Li J, Guariglia S, Meng Q, Cai D
Neuron (2011) 693: 523-35. 105 043 IHC

Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM

Cat. No.: 105 043

Amount: 50 µg

Price: $375.00

Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM

Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin4 is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
The localization of synaptotagmin4 is still under discussion. A localization to synaptic vesicles (SVs) has been postulated but more recent studies suggest that it is present in the Golgi compartment, in distal parts of neurites and on large dense core vesicles (LDCVs) of NGF differentiated PC12 cells.

1 ) Ferguson GD et al., Hippocampus (2004)
2 ) Dai H et al., Nat. Struct. Mol. Biol. (2004)
3 ) Ferguson GD et al., Neuropharmacology (2004)
4 ) Zhang Q et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
5 ) Fukuda M et al., J. Biol. Chem. (2003)
6 ) Ibata K et al., Neurosci. Res. (2002)
7 ) Ferguson GD et al., Mol. Neurobiol. ()
8 ) Fukuda M et al., J. Neurochem. (2001)
9 ) Ibata K et al., J. Neurochem. (2000)
10 ) Thomas DM et al., Mol. Biol. Cell (1999)

Protocols

Standard protocol

OTHER

Preabsorbtion

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Synaptotagmin4 antibody - 105 043 K.O.

References for Synaptotagmin4 - 105 043