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Home > Products > 103 002

Synaptogyrin1 antibody - 103 002 K.O.

Synaptogyrin is a major synaptic vesicle membrane protein; tyrosine-phosphorylated
Rabbit polyclonal antiserum
Cat. No.: 103 002
Amount: 200 µl
Price: $355.00
Cat. No. 103 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen Synthetic peptide corresponding to AA 220 to 234 from rat Synaptogyrin1 (UniProt Id: Q62876)
Reactivity Reacts with: human (O43759), rat (Q62876), mouse (O55100), hamster, chicken.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 31090538
Matching control protein/peptide 103-0P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 103 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 103_002.pdf

References for Synaptogyrin1 - 103 002

Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence.
Choi I, Wang M, Yoo S, Xu P, Seegobin SP, Li X, Han X, Wang Q, Peng J, Zhang B, Yue Z, et al.
Nature cell biology (2023) 257: 963-974. 103 002 WB; tested species: mouse
Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca2+ Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons.
Vevea JD, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4319: 3421-3438. 103 002 WB; tested species: rat
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 103 002 WB; tested species: mouse
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife (2020) 9: . 103 002 WB; tested species: mouse
Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
De Pace R, Britt DJ, Mercurio J, Foster AM, Djavaherian L, Hoffmann V, Abebe D, Bonifacino JS
Cell reports (2020) 3111: 107775. 103 002 WB; tested species: mouse
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 103 002 WB; KO verified; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 103 002 WB
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 103 002 WB; tested species: rat
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 103 002 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 103 002 ICC, IHC; tested species: mouse,rat
Lowering Synaptogyrin-3 expression rescues Tau-induced memory defects and synaptic loss in the presence of microglial activation.
Largo-Barrientos P, Apóstolo N, Creemers E, Callaerts-Vegh Z, Swerts J, Davies C, McInnes J, Wierda K, De Strooper B, Spires-Jones T, de Wit J, et al.
Neuron (2021) : . 103 002 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 103 002 ICC, IHC; tested species: mouse,rat
Cat. No.: 103 002
Amount: 200 µl
Price: $355.00
Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence.
Choi I, Wang M, Yoo S, Xu P, Seegobin SP, Li X, Han X, Wang Q, Peng J, Zhang B, Yue Z, et al.
Nature cell biology (2023) 257: 963-974. 103 002 WB; tested species: mouse
Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca2+ Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons.
Vevea JD, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4319: 3421-3438. 103 002 WB; tested species: rat
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 103 002 WB; tested species: mouse
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife (2020) 9: . 103 002 WB; tested species: mouse
Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
De Pace R, Britt DJ, Mercurio J, Foster AM, Djavaherian L, Hoffmann V, Abebe D, Bonifacino JS
Cell reports (2020) 3111: 107775. 103 002 WB; tested species: mouse
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 103 002 WB; KO verified; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 103 002 WB
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 103 002 WB; tested species: rat
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 103 002 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 103 002 ICC, IHC; tested species: mouse,rat
Lowering Synaptogyrin-3 expression rescues Tau-induced memory defects and synaptic loss in the presence of microglial activation.
Largo-Barrientos P, Apóstolo N, Creemers E, Callaerts-Vegh Z, Swerts J, Davies C, McInnes J, Wierda K, De Strooper B, Spires-Jones T, de Wit J, et al.
Neuron (2021) : . 103 002 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 103 002 ICC, IHC; tested species: mouse,rat
WB
ICC
IHC
IHC-P
Background

Synaptogyrins are tyrosine-phosphorylated proteins with two neuronal (synaptogyrin 1 and 3) and one ubiquitous, synaptogyrin 2 or cellugyrin isoform.
Synaptogyrins are integral membrane proteins and localize to the membrane of small vesicles. Synaptogyrin 1 and 3 are expressed in the brain whereby the latter shows a more restricted expression pattern with high levels in the mossy fiber region of the hippocampus, substantia nigra pars reticulata, pallidum, and deep cerebellar nuclei.
Synaptogyrin 2/cellugyrin, a close relative, is expressed in all tissues, for instance, in distinct populations of GLUT 4 containing vesicles.
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