Tailor-made Antibodies
and Tools for Life Science

Ribeye antibody A-domain - 192 103 K.O.

Ribeye is a scaffolding protein of the ribbon synapse
Rabbit polyclonal purified antibody
Cat. No.: 192 103
Amount: 50 µg
Price: $385.00
Cat. No. 192 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC-P: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 95 to 207 from rat Ribeye (UniProt Id: Q9EQH5-2)
Reactivity Reacts with: rat (Q9EQH5-2), mouse (P56546-2), human (P56545-2).
Other species not tested yet.
Specificity This antibody recognizes only ribeye and not CtBP 2. K.O. validated PubMed: 35742873
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.

Data sheet 192_103.pdf

References for Ribeye - 192 103

RIBEYE B-Domain Is Essential for RIBEYE A-Domain Stability and Assembly of Synaptic Ribbons.
Shankhwar S, Schwarz K, Katiyar R, Jung M, Maxeiner S, Südhof TC, Schmitz F
Frontiers in molecular neuroscience (2022) 15: 838311. 192 103 WB; tested species: mouse
α2δ-4 is required for the molecular and structural organization of rod and cone photoreceptor synapses.
Kerov V, Laird JG, Joiner ML, Knecht S, Soh D, Hagen J, Gardner SH, Gutierrez W, Yoshimatsu T, Bhattarai S, Puthussery T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 192 103 WB, IHC; tested species: mouse
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 192 103 WB; tested species: rat
Cat. No.: 192 103
Amount: 50 µg
Price: $385.00