Synaptic Systems

Cat. No.: 192 003

Amount: 50 µg

Price: $375.00

Cat. No. 192 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 10000 (see remarks) gallery IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 974 to 988 from rat Ribeye (UniProt Id: Q9EQH5-2)
Reactivity	Reacts with: human (P56545-2), rat (Q9EQH5), mouse (P56546-2), monkey, cow. Other species not tested yet.
Specificity	This antibody recognizes ribeye and CtBP 2 (cat. no. 193 003).
Remarks	The immunogen is identical to that of anti-CtBP 2 (cat. no. 193 003). IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet	192_003.pdf

References for Ribeye - 192 003

Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance.
Hagiwara A, Kitahara Y, Grabner CP, Vogl C, Abe M, Kitta R, Ohta K, Nakamura K, Sakimura K, Moser T, Nishi A, et al.
The Journal of cell biology (2018) : . 192 003 WB, IHC; tested species: mouse

Neuronal p58IPK Protects Retinal Ganglion Cells Independently of Macrophage/Microglia Activation in Ocular Hypertension.
McLaughlin T, Wang J, Jia L, Wu F, Wang Y, Wang JJ, Mu X, Zhang SX
Cells (2023) 1212: . 192 003 IHC; tested species: mouse

The rod synapse in aging wildtype and Dscaml1 mutant mice.
Clemons MR, Dimico RH, Black C, Schlussler MK, Camerino MJ, Aldinger-Gibson K, Bartle A, Reynolds N, Eisenbrandt D, Rogers A, Andrianu J, et al.
PloS one (2023) 1811: e0290257. 192 003 IHC; tested species: mouse

Critical Role of the Presynaptic Protein CAST in Maintaining the Photoreceptor Ribbon Synapse Triad.
Hagiwara A, Mizutani A, Kawamura S, Abe M, Hida Y, Sakimura K, Ohtsuka T
International journal of molecular sciences (2023) 248: . 192 003 IHC; tested species: mouse

Essential Role of XBP1 in Maintaining Photoreceptor Synaptic Integrity in Early Diabetic Retinopathy.
McLaughlin T, Wang G, Medina A, Perkins J, Nihlawi R, Seyfried D, Hu Z, Wang JJ, Zhang SX
Investigative ophthalmology & visual science (2023) 6414: 40. 192 003 IHC; tested species: mouse

Overloaded Adeno-Associated Virus as a Novel Gene Therapeutic Tool for Otoferlin-Related Deafness.
Rankovic V, Vogl C, Dörje NM, Bahader I, Duque-Afonso CJ, Thirumalai A, Weber T, Kusch K, Strenzke N, Moser T
Frontiers in molecular neuroscience (2020) 13: 600051. 192 003 IHC; tested species: mouse

Loss of XBP1 Leads to Early-Onset Retinal Neurodegeneration in a Mouse Model of Type I Diabetes.
McLaughlin T, Siddiqi M, Wang JJ, Zhang SX
Journal of clinical medicine (2019) 86: . 192 003 IHC; tested species: mouse

Loss of XBP1 accelerates age-related decline in retinal function and neurodegeneration.
McLaughlin T, Falkowski M, Park JW, Keegan S, Elliott M, Wang JJ, Zhang SX
Molecular neurodegeneration (2018) 131: 16. 192 003 IHC; tested species: mouse

Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina.
Farshi P, Fyk-Kolodziej B, Krolewski DM, Walker PD, Ichinose T
The Journal of comparative neurology (2016) 52410: 2059-79. 192 003 IHC; tested species: mouse

A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 192 003 IHC

LKB1 and AMPK regulate synaptic remodeling in old age.
Samuel MA, Voinescu PE, Lilley BN, de Cabo R, Foretz M, Viollet B, Pawlyk B, Sandberg MA, Vavvas DG, Sanes JR
Nature neuroscience (2014) 179: 1190-7. 192 003 IHC; tested species: mouse

Cat. No.: 192 003

Amount: 50 µg

Price: $375.00

IHC

Background

The photoreceptor ribbon synapse is a unique type of synapse specialized for the tonic release of neurotransmitter in the dark. Ribeye is a self-aggregating protein and is one of the major scaffolding components of the ribbon on which the neurotransmitter containing vesicles are tethered. The protein consists of a unique A-domain and a B-domain. With the exception of the first 20 amino acids the B-domain is identical to the transcriptional corepressor CtBP 2. Both proteins originate from the same gene.

1 ) Verger A et al., Mol. Cell. Biol. (2006)
2 ) Brandt A et al., J. Neurosci. (2005)
3 ) tom Dieck S et al., J. Cell Biol. (2005)
4 ) Schmitz F et al., Neuron (2000)
5 ) Furusawa T et al., Mol. Cell. Biol. (1999)

Protocols

IHC

IHC-P

Other products for this target

Ribeye antibody B-domain - 192 003

References for Ribeye - 192 003