Synaptic Systems

Cat. No.: 390 005

Amount: 50 µg

Price: $470.00

Cat. No. 390 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)
Specificity	Recognizes mRFP, mCherry, mOrgange2, dsRed, tdTomato, mScarlet.
Data sheet	390_005.pdf

References for RFP - 390 005

Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
Concina G, Milano L, Renna A, Manassero E, Stabile F, Sacchetti B
Cell reports (2024) 435: 114151. 390 005 IHC; tested species: rat

Individual thalamic inhibitory interneurons are functionally specialized toward distinct visual features.
Müllner FE, Roska B
Neuron (2024) 11216: 2765-2782.e9. 390 005 IHC; tested species: mouse

Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments.
Ogawa Y, Lim BC, George S, Oses-Prieto JA, Rasband JM, Eshed-Eisenbach Y, Hamdan H, Nair S, Boato F, Peles E, Burlingame AL, et al.
Nature communications (2023) 141: 6797. 390 005 IHC; tested species: rat

Purkinje cell microzones mediate distinct kinematics of a single movement.
Blot FGC, White JJ, van Hattem A, Scotti L, Balaji V, Adolfs Y, Pasterkamp RJ, De Zeeuw CI, Schonewille M
Nature communications (2023) 141: 4358. 390 005 IHC; tested species: mouse

Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S.
Asencor AI, Dvoryanchikov G, Makhoul V, Tsoulfas P, Chaudhari N
eNeuro () 93: . 390 005 IHC; tested species: mouse

Cat. No.: 390 005

Amount: 50 µg

Price: $470.00

Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S.
Asencor AI, Dvoryanchikov G, Makhoul V, Tsoulfas P, Chaudhari N
eNeuro () 93: . 390 005 IHC; tested species: mouse

ICC

IHC

Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D et al., Genesis (2010)
2 ) Shaner NC et al., Nat. Biotechnol. (2004)
3 ) Labas YA et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
4 ) Fradkov AF et al., FEBS Lett. (2000)

Protocols

ICC

IHC

Other products for this target

RFP antibody - 390 005

References for RFP - 390 005