Synaptic Systems

Cat. No.: 107 303

Amount: 50 µg

Price: $375.00

Cat. No. 107 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 3 to 16 and 193 to 210 from rat Rab3d (UniProt Id: Q63942)
Reactivity	Reacts with: rat (Q63942), mouse (P35276). Other species not tested yet.
Remarks	This antibody has been purified over a full-length Rab 3d column.
Data sheet	107_303.pdf

References for Rab3d - 107 303

Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis.
Gustafsson JK, Davis JE, Rappai T, McDonald KG, Kulkarni DH, Knoop KA, Hogan SP, Fitzpatrick JA, Lencer WI, Newberry RD
eLife (2021) 10: . 107 303 IHC; tested species: mouse

Cat. No.: 107 303

Amount: 50 µg

Price: $375.00

IHC-P

Background

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca²⁺ regulated excocytosis and converts it into an constitutive Ca²⁺ independent exocytosis mechanism.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Geppert M et al., Nature (1997)
4 ) Südhof TC et al., Nature (1995)
5 ) Fischer von Mollard G et al., Eur. J. Cell Biol. (1994)
6 ) Chen X et al., J. Biol. Chem. (2003)
7 ) Millar AL et al., Histol. Histopathol. (2002)
8 ) Pavlos NJ et al., J. Mol. Endocrinol. (2001)
9 ) Lin CG et al., Biochem. J. (1997)
10 ) Fischer von Mollard G et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

IHC-P

Rab3d antibody - 107 303

References for Rab3d - 107 303