Synaptic Systems

Cat. No.: 168-0P

Amount: 100 µg

Price: $105.00

Background

Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.

1 ) Mahoney TR et al., Mol. Biol. Cell (2006)
2 ) Jordens I et al., Pigment Cell Res. (2006)
3 ) Tsuboi T et al., J. Cell. Sci. (2006)
4 ) Aslan D et al., Pediatr Hematol Oncol ()
5 ) Izumi T et al., Meth. Enzymol. (2005)
6 ) Kasai K et al., J. Clin. Invest. (2005)
7 ) Aizawa T et al., J. Clin. Invest. (2005)
8 ) Chen X et al., Biochem. Biophys. Res. Commun. (2004)
9 ) Westbroek W et al., Pigment Cell Res. (2004)
10 ) Imai A et al., J. Cell. Sci. (2004)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 168-0P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	168 003, 168 013
Remarks	This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	168-0p.pdf

Rab27A protein - 168-0P