Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

PSD95 antibody - 124 003 K.O.

PSD 95 is a component of postsynaptic densities in central synapses
Rabbit polyclonal purified antibody
Cat. No.: 124 003
Amount: 50 µg
Price: $460.00
Cat. No. 124 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 18 to 32 from rat PSD95 (UniProt Id: P31016)
Reactivity Reacts with: rat (P31016), mouse (Q62108).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 124-0P
Data sheet 124_003.pdf

References for PSD95 - 124 003

Visual Cortex Engagement in Retinitis Pigmentosa.
Pietra G, Bonifacino T, Talamonti D, Bonanno G, Sale A, Galli L, Baroncelli L
International journal of molecular sciences (2021) 2217: . 124 003 WB; tested species: mouse
The Different Molecular Code in Generation of Dopaminergic Neurons from Astrocytes and Mesenchymal Stem Cells.
Wang N, Ji X, Wu Y, Zhou S, Peng H, Wang J, Yu S, Zhang J
International journal of molecular sciences (2021) 2222: . 124 003 WB; tested species: rat
Age-related deficits in neuronal physiology and cognitive function are recapitulated in young mice overexpressing the L-type calcium channel, CaV 1.3.
Moore SJ, Cazares VA, Temme SJ, Murphy GG
Aging cell (2023) 223: e13781. 124 003 ICC; tested species: mouse
Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 124 003 ICC; tested species: mouse
The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 124 003 IHC; tested species: mouse
Age-Dependent Regulation of Dendritic Spine Density and Protein Expression in Mir324 KO Mice.
Parkins EV, Burwinkel JM, Ranatunga R, Yaser S, Hu YC, Tiwari D, Gross C
Journal of molecular neuroscience : MN (2023) 739-10: 818-830. 124 003 IHC; tested species: mouse
Vascularized human cortical organoids (vOrganoids) model cortical development in vivo.
Shi Y, Sun L, Wang M, Liu J, Zhong S, Li R, Li P, Guo L, Fang A, Chen R, Ge WP, et al.
PLoS biology (2020) 185: e3000705. 124 003 IHC; tested species: mouse
Functional Neuroligin-2-MDGA1 interactions differentially regulate synaptic GABAARs and cytosolic gephyrin aggregation.
Zeppillo T, Ali H, Ravichandran S, Ritter TC, Wenger S, López-Murcia FJ, Gideons E, Signorelli J, Schmeisser MJ, Wiltfang J, Rhee J, et al.
Communications biology (2024) 71: 1157. 124 003 IHC-Fr; tested species: mouse
Cat. No.: 124 003
Amount: 50 µg
Price: $460.00
Visual Cortex Engagement in Retinitis Pigmentosa.
Pietra G, Bonifacino T, Talamonti D, Bonanno G, Sale A, Galli L, Baroncelli L
International journal of molecular sciences (2021) 2217: . 124 003 WB; tested species: mouse
The Different Molecular Code in Generation of Dopaminergic Neurons from Astrocytes and Mesenchymal Stem Cells.
Wang N, Ji X, Wu Y, Zhou S, Peng H, Wang J, Yu S, Zhang J
International journal of molecular sciences (2021) 2222: . 124 003 WB; tested species: rat
Age-related deficits in neuronal physiology and cognitive function are recapitulated in young mice overexpressing the L-type calcium channel, CaV 1.3.
Moore SJ, Cazares VA, Temme SJ, Murphy GG
Aging cell (2023) 223: e13781. 124 003 ICC; tested species: mouse
Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 124 003 ICC; tested species: mouse
The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 124 003 IHC; tested species: mouse
Age-Dependent Regulation of Dendritic Spine Density and Protein Expression in Mir324 KO Mice.
Parkins EV, Burwinkel JM, Ranatunga R, Yaser S, Hu YC, Tiwari D, Gross C
Journal of molecular neuroscience : MN (2023) 739-10: 818-830. 124 003 IHC; tested species: mouse
Vascularized human cortical organoids (vOrganoids) model cortical development in vivo.
Shi Y, Sun L, Wang M, Liu J, Zhong S, Li R, Li P, Guo L, Fang A, Chen R, Ge WP, et al.
PLoS biology (2020) 185: e3000705. 124 003 IHC; tested species: mouse
Functional Neuroligin-2-MDGA1 interactions differentially regulate synaptic GABAARs and cytosolic gephyrin aggregation.
Zeppillo T, Ali H, Ravichandran S, Ritter TC, Wenger S, López-Murcia FJ, Gideons E, Signorelli J, Schmeisser MJ, Wiltfang J, Rhee J, et al.
Communications biology (2024) 71: 1157. 124 003 IHC-Fr; tested species: mouse
Background

PSD 95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.