Piccolo antibody - 142 116

Piccolo is a large multi-domain protein of the presynaptic active zone

Chicken polyclonal IgY fraction

Cat. No.: 142 116

Amount: 200 µl

Price: $365.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 142 116	200 µl antibody, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery ExM: 1 : 100 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to a central region of rat piccolo (UniProt Id: Q9JKS6)
Reactivity	Reacts with: rat (Q9JKS6), mouse (Q9QYX7). Other species not tested yet.
Remarks	ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet	142_116.pdf

References for Piccolo - 142 116

Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties.
Silm K, Yang J, Marcott PF, Asensio CS, Eriksen J, Guthrie DA, Newman AH, Ford CP, Edwards RH
Neuron (2019) : . 142 116 ICC; tested species: mouse

Cat. No.: 142 116

Amount: 200 µl

Price: $365.00

ICC

IHC

IHC-P

ExM

Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn²⁺ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

1 ) Shapira M et al., Neuron (2003)
2 ) Fenster SD et al., J. Biol. Chem. (2003)
3 ) Fenster SD et al., Int. J. Dev. Neurosci. ()
4 ) Dick O et al., J. Comp. Neurol. (2001)
5 ) Gerber SH et al., EMBO J. (2001)
6 ) Fenster SD et al., Neuron (2000)
7 ) Cases-Langhoff C et al., Eur. J. Cell Biol. (1996)

Protocols

ICC

IHC

IHC-P

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