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Home > Products > 195 011C3

Parvalbumin antibody - 195 011C3

Parvalbumin is calcium binding protein specific for a subpopulation of GABAergic interneurones
Mouse monoclonal purified IgG
Cat. No.: 195 011C3
Amount: 100 µg
Price: $465.00
Cat. No. 195 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Clone 58E1
Subtype IgG1 (κ light chain)
Immunogen Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity Reacts with: rat (P02625), mouse (P32848).
No signal: zebrafish.
Other species not tested yet.
Matching control protein/peptide 195-0P
Data sheet 195_011c3.pdf

References for Parvalbumin - 195 011C3

Brain volumetric alterations accompanied with loss of striatal medium-sized spiny neurons and cortical parvalbumin expressing interneurons in Brd1+/- mice.
Qvist P, Eskildsen SF, Hansen B, Baragji M, Ringgaard S, Roovers J, Paternoster V, Molgaard S, Corydon TJ, Stødkilde-Jørgensen H, Glerup S, et al.
Scientific reports (2018) 81: 16486. 195 011C3 IHC; tested species: mouse
Cat. No.: 195 011C3
Amount: 100 µg
Price: $465.00
Brain volumetric alterations accompanied with loss of striatal medium-sized spiny neurons and cortical parvalbumin expressing interneurons in Brd1+/- mice.
Qvist P, Eskildsen SF, Hansen B, Baragji M, Ringgaard S, Roovers J, Paternoster V, Molgaard S, Corydon TJ, Stødkilde-Jørgensen H, Glerup S, et al.
Scientific reports (2018) 81: 16486. 195 011C3 IHC; tested species: mouse
IHC
Background

Parvalbumin is a small, acidic calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurons. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.
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