Synaptic Systems

Cat. No.: 196 003

Amount: 50 µg

Price: $455.00

Cat. No. 196 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 293 to 384 from mouse PACSIN1 (UniProt Id: Q61644)
Reactivity	Reacts with: rat (Q9Z0W5), mouse (Q61644), human (Q9BY11), zebrafish. Other species not tested yet.
Specificity	K.D. validated PubMed: 30683896
Matching control protein/peptide	196-0P
Data sheet	196_003.pdf

References for PACSIN1 - 196 003

Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, et al.
Nature communications (2019) 101: 428. 196 003 WB, IHC; KD verified; tested species: human,zebrafish

Cat. No.: 196 003

Amount: 50 µg

Price: $455.00

ICC

Background

This protein has been described as PACSIN 1 (for PKC and CK2 substrate in neurons) and syndapin 1 (synaptic, dynamin-associated protein I). It is a multidomain phospho-protein that interacts via its SH3 domain with dynamin 1, synaptojanin, synapsin and N-WASP. Since N-WASP is an activator of the Arp2/3 complex, syndapin may link membrane trafficking and the actin cytoskeleton.
Up to now, 3 isoforms have been described. Syndapin 1 shows a neuronal, syndapin 3 a muscular and syndapin 2 an ubiquitous expression profile.

1 ) Anggono V et al., Nat. Neurosci. (2006)
2 ) Kessels MM et al., J. Biol. Chem. (2006)
3 ) Kessels MM et al., J. Cell. Sci. (2004)
4 ) Modregger J et al., J. Cell. Sci. (2000)
5 ) Qualmann B et al., J. Cell Biol. (2000)
6 ) Qualmann B et al., Mol. Biol. Cell (1999)
7 ) Plomann M et al., Eur. J. Biochem. (1998)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

PACSIN1 antibody - 196 003 K.D.

References for PACSIN1 - 196 003