Proton ATPase peptide 116 kDa subunit - 109-0P

VOa1 is the largest subunit of the vacuolar proton pump in synaptic vesicles and coated vesicles

control peptide

Cat. No.: 109-0P

Amount: 100 µg

Price: $105.00

Cat. No. 109-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 826 to 838 from rat Proton ATPase (UniProt Id: P25286)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	109 002, 109 003
Remarks	This control peptide consists of the synthetic peptide (FSFEHIREGKFDE) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	109-0p.pdf

Cat. No.: 109-0P

Amount: 100 µg

Price: $105.00

Background

The Proton ATPase, also referred to as vacuolar proton pump, is involved in the acidification of many intracellular organelles. The pump is composed of more than 10 subunits, of which the 116 kDa subunit is the largest. This subunit has an N-terminal cytoplasmic domain and a C-terminal transmembrane domain with probably 6 transmembrane regions. The 116 kDa subunit is essential for proton pump activity.

1 ) Südhof TC et al., Nature (1995)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Perin MS et al., J. Biol. Chem. (1991)

Protocols

OTHER

Preadsorbtion

Other products for this target