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Home > Products > 109 003

Proton ATPase antibody 116 kDa subunit - 109 003 K.D.

VOa1 is the largest subunit of the vacuolar proton pump in synaptic vesicles and coated vesicles
Rabbit polyclonal purified antibody
Cat. No.: 109 003
Amount: 50 µg
Price: $455.00
Cat. No. 109 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 (see remarks) gallery  
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 826 to 838 from rat Proton ATPase (UniProt Id: P25286)
Reactivity Reacts with: rat (P25286), mouse (Q9Z1G4), hamster.
No signal: fish.
Other species not tested yet.
Specificity Specific for the α1 116kDa subunit. K.D. validated
Matching control protein/peptide 109-0P
Remarks

WB:  Proton ATPase aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: Methanol fixation is recommended.
IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
 
Data sheet 109_003.pdf

References for Proton ATPase - 109 003

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 109 003 WB; tested species: mouse
Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 109 003 WB; tested species: mouse
ATP6V0d2 controls Leishmania parasitophorous vacuole biogenesis via cholesterol homeostasis.
Pessoa CC, Reis LC, Ramos-Sanchez EM, Orikaza CM, Plaza CC, de Castro Levatti EV, Badaró ACB, Yamamoto JUDS, D'Almeida V, Goto H, Mortara RA, et al.
PLoS pathogens (2019) 156: e1007834. 109 003 WB; tested species: mouse
Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.
Farsi Z, Gowrisankaran S, Krunic M, Rammner B, Woehler A, Lafer EM, Mim C, Jahn R, Milosevic I
eLife (2018) 7: . 109 003 WB; tested species: mouse
Cat. No.: 109 003
Amount: 50 µg
Price: $455.00
The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 109 003 WB; tested species: mouse
Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 109 003 WB; tested species: mouse
ATP6V0d2 controls Leishmania parasitophorous vacuole biogenesis via cholesterol homeostasis.
Pessoa CC, Reis LC, Ramos-Sanchez EM, Orikaza CM, Plaza CC, de Castro Levatti EV, Badaró ACB, Yamamoto JUDS, D'Almeida V, Goto H, Mortara RA, et al.
PLoS pathogens (2019) 156: e1007834. 109 003 WB; tested species: mouse
Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.
Farsi Z, Gowrisankaran S, Krunic M, Rammner B, Woehler A, Lafer EM, Mim C, Jahn R, Milosevic I
eLife (2018) 7: . 109 003 WB; tested species: mouse
WB
ICC
IHC
Background
The Proton ATPase, also referred to as vacuolar proton pump, is involved in the acidification of many intracellular organelles. The pump is composed of more than 10 subunits, of which the 116 kDa subunit is the largest. This subunit has an N-terminal cytoplasmic domain and a C-terminal transmembrane domain with probably 6 transmembrane regions. The 116 kDa subunit is essential for proton pump activity.
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